Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Human Integrin aL/CD11a Monoclonal Antibody (Catalog # MAB3595) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin aL/CD11a at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Integrin alphaL/CD11a by Simple Western^TM.

Simple Western lane view shows lysates of PBMC, Placenta, loaded at 0.2 mg/mL. A specific band was detected for Integrin alphaL/CD11a at approximately 220 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Integrin alphaL/CD11a Monoclonal Antibody (Catalog # MAB3595). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Integrin alphaL/CD11a Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells

Simple Western

20 µg/mL
Sample: PBMC, Placenta

Western Blot

1 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMC) under non-reducing conditions only

Reviewed Applications

Read 1 review rated 4 using MAB3595 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha L/CD11a

Integrin subunit alphaL/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin beta2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin alphaL contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alphaL ECD share 74% aa identity. A second isoform has a 53 aa insert in the ECD.

Alternate Names

CD11a, ITGAL, LFA-1 alpha, Ly-15, Ly-21

Entrez Gene IDs

3683 (Human); 16408 (Mouse); 308995 (Rat)

Gene Symbol

ITGAL

UniProt

CAA68747

Additional Integrin alpha L/CD11a Products

Product Documents for Human Integrin alphaL/CD11a Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Integrin alphaL/CD11a Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin alphaL/CD11a Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3595

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Integrin alpha L/CD11a Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Integrin alphaL/CD11a Antibody

Detection of Human Integrin alphaL/CD11a by Western Blot.

Detection of Human Integrin alphaL/CD11a by Simple WesternTM.