Human IL-32 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC30402A
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 373821
Product Specifications
Immunogen
E. coli-derived recombinant human IL-32 alpha
Met1-Asn131
Accession # P24001
Met1-Asn131
Accession # P24001
Specificity
Detects human IL-32 alpha and other IL-32 isoforms in direct ELISAs. In direct ELISAs, 30% cross-reactivity with recombinant human (rh) IL-32 beta and 38% cross-reactivity with rhIL-32 gamma is detected.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human IL-32 APC-conjugated Antibody
Detection of IL‑32 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin were stained with Rat Anti-Human IL-32 APC-conjugated Monoclonal Antibody (Catalog # IC30402A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human IL-32 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Ca2+ Ionomycin and fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Ca2+ Ionomycin and fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: IL-32
IL-32 alpha is one of at least nine potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4), which when full-length is 31 kDa in size. The predicted molecular weight of IL-32 alpha is approximately 15 kDa and it represents amino acids (aa) 1-18, 65-153, and 211-234 of the full-length (234 aa) form. Since the signal sequence of the ORF is aa 1-30, this isofom is like found intracellularly. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN‑ gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn Induces cytokine expression. No ortholog has been found in mouse.
Long Name
Interleukin 32
Alternate Names
IL32, NK4, TAIF
Entrez Gene IDs
9235 (Human)
Gene Symbol
IL32
UniProt
Additional IL-32 Products
Product Documents for Human IL-32 APC-conjugated Antibody
Product Specific Notices for Human IL-32 APC-conjugated Antibody
For research use only
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