Detects human IL‑13 R alpha1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinat human (rh) IL-13 R alpha2, recombinant mouse IL-13 R alpha1, rhIL-5 R alpha, rhIL-5 R beta, rhIL-4 R, and rhIL-9 R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-13 R alpha1 Antibody

Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 R alpha1 Antibody.

Recombinant Human IL-13 (213-ILB) stimulates prolif-eration in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-13 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152). The ND₅₀ is typically 1.00-12.0 µg/mL.

Detection of IL-13 R alpha1 in Human Granulocytes by Flow Cytometry.

Human whole blood granulocytes were stained with Goat Anti-Human IL-13 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152, filled histogram) or control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107).

IL‑13 R alpha1 in Human Skin.

IL-13 Ra1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL-13 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

IL-13 R alpha1 in Human Heart.

IL-13 R alpha1 was detected in immersion fixed paraffin-embedded sections of Human Heart using Goat Anti-Human IL-13 R alpha1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in myocardial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of IL-13 R alpha 1 by Western Blot

IL-4R alpha drives IL-4- and IL-13-mediated signal transduction.N1 immortalized human prostate fibroblasts were treated with vehicle, IL-4 (20ng/ml), or IL-13 (20 ng/m) with or without 2 hr pre-treatment with IL4R alpha (IL4R) (400 ng) or IL-13R alpha1 (40 ng) antibodies. Both IL-4 (Fig 7A) and IL-13 (Fig 7D) robustly and significantly induced STAT6 phosphoryation compared to vehicle-treated cells. IL-4 stimulation of STAT6 phosphorylation was repressed upon pre-treatment with IL4R alpha (A) but not IL-13R alpha1 (B) antibodies, whereas IL-13 stimulation of STAT6 phosphorylation was repressed upon pre-treatment with either IL-13R alpha1 (D) or IL4R alpha (E) antibodies. Densitometric data (S7 Fig) from the replicate experiments is graphed in C (IL-4) and F (IL-13). Significant differences are indicated as * p <.05; ** p <.01; *** p <.001; **** p <.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36201508), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IL-13 R alpha 1 by Western Blot

Human IL-13 R alpha 1 ELISA Standard Curve

Recombinant Human IL-13 R alpha1 Fc Chimera (Catalog # 146-IR) was serially diluted and captured by Mouse Anti-Human IL-13 R alpha1 Monoclonal Antibody (Catalog # MAB146) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-13 R alpha1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

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Applications for Human IL-13 R alpha1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human whole blood granulocytes

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin and human heart.

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL-13 R alpha1 Fc Chimera (Catalog # 146-IR)

Neutralization

Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 1.00-12.0 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑13.

Reviewed Applications

Read 2 reviews rated 3 using AF152 in the following applications:

Block/Neutralize (1 Review)
Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13 R alpha 1

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha' or NR4, is now referred to as IL-13 R alpha1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha', is now referred to as IL-13 R alpha2. The human IL-13 R alpha1 was originally cloned based on sequence homology to the mouse IL-13 R alpha1. The IL‑13 R alpha1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha1 share 76% aa sequence identity. The extracellular domain of IL‑13 R alpha1 is also closely related to that of IL-13 R alpha2. IL-13 R alpha1 has been shown to combine with the IL-4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha2 in IL-13 signaling remains to be elucidated.

References

Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.

Long Name

Interleukin 13 Receptor alpha 1

Alternate Names

CD213a1, IL-13Ra1, IL13R alpha 1, IL13RA1

Entrez Gene IDs

3597 (Human); 16164 (Mouse)

Gene Symbol

IL13RA1

UniProt

Q5JSL4

Additional IL-13 R alpha 1 Products

Product Documents for Human IL-13 R alpha1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human IL-13 R alpha1 Antibody

For research use only

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Calculators
Flow Cytometry