Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all ID2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ID2
Met1-Gly134
Accession # Q02363

Specificity

Detects human ID2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ID1, rhID3, and rhID4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ID2 Antibody

ID2 antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

ID2 in A549 Human Cell Line.

ID2 was detected in immersion fixed A549 human lung carcinoma cell line using 10 µg/mL Human ID2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4660) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human ID2 antibody by Western Blot.

Detection of Human ID2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human ID2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4660) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ID2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human ID2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ID2

ID2 (Inhibitor of DNA binding 2; also known as inhibitor of differentiation 2) is a 15 kDa member of the small ID HLH family of proteins. It is a nuclear protein that forms inactivating heterodimers with bHLH transcription factors. bHLH proteins activate lineage-specific genes and ID2. Human ID2 is 134 amino acids (aa) in length. It contains one HLH domain (aa 36-76) but no DNA-binding motif. Full-length human ID2 is 98% aa identical to both mouse and porcine ID2.

Long Name

Inhibitor of DNA Binding 2

Alternate Names

GIG8

Entrez Gene IDs

3398 (Human); 15902 (Mouse); 25587 (Rat)

Gene Symbol

ID2

UniProt

Q02363

Additional ID2 Products

Product Documents for Human ID2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ID2 Antibody

For research use only

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