Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Host Cell Factor 1/HCFC1 at approximately 300 kDa (as indicated) and proteolytic fragments of Host Cell Factor 1/HCFC1 at approximately 100 to 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Host Cell Factor 1/HCFC1 in HeLa Human Cell Line.

Host Cell Factor 1/HCFC1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Host Cell Factor 1/HCFC1 in Human Colon Cancer Tissue.

Host Cell Factor 1/HCFC1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Host Cell Factor 1/HCFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6254) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Host Cell Factor 1/HCFC1 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Host Cell Factor 1/HCFC1

HCF-1 (Host cell factor; also called C1 factor and VCAF) is a blanket name for a group of polypeptides that are generated through the targeted proteolysis of a large 300 kDa transcriptional coactivator precursor. The precursor is widely expressed, and its products are posited to participate in multiple activities, such as mRNA processing, transcriptional coactivation, and cell cycle progression through G0/G1 transition. Human HCF-1 is 2035 amino acids (aa) in length. It is modular in structure, and contains a Kelch-repeat region (aa 32-313), an SP1/GABP basic binding sequence (aa 478-875), an acidic transactivation domain (aa 1530-1735), and a C-terminal NLS-containing Trp/Tyr/Phe-rich region (aa 1760-2035). Situated between the basic and acidic regions is an HCF repeat region (aa 1010-1439) that contains six 26 aa repeats that undergo autocatalytic cleavage. HCF-1 is O-glycosylated, phosphorylated and acetylated. In quiescent cells, the full-length 300 kDa precursor can be found in the cytoplasm, along with a presumed 50 kDa fragment that appears to represent some sequence between aa 300-1000. In activated cells, HCF-1 appears in the nucleus, and undergoes autocatalysis at one or more sites occurs distal to Glu at position 1019, 1081, 1110, 1295, 1323, and 1423. This generates multiple fragments that noncovalently associate with each other and appear as bands in SDS-Page that range from 100-175 kDa in size. This association is quite vigorous and requires aggressive treatment to achieve denaturation. There is one potential isoform that contains an alternative start site at Met100, a second isoform that shows a deletion of aa 1072-1101, a third isoform that exhibits a Leu substitution for aa 428-2035, and a final isoform that shows a deletion of aa 382-450. Over aa 1626-1836, human HCF-1 shares 95% aa identity with mouse HCF-1.

Alternate Names

C1 Factor, CFF, HCF1, HFC1

Entrez Gene IDs

3054 (Human); 15161 (Mouse); 363519 (Rat)

Gene Symbol

HCFC1

UniProt

P51610 (Human)

Additional Host Cell Factor 1/HCFC1 Products

Product Documents for Human Host Cell Factor 1/HCFC1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Host Cell Factor 1/HCFC1 Antibody

For research use only

Citations
Reviews

Human Host Cell Factor 1/HCFC1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6254

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Host Cell Factor 1/HCFC1 Antibody

Detection of Human Host Cell Factor 1/HCFC1 by Western Blot.