Human GRB2 (SH2 Domain) Alexa Fluor™ Plus 680-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB38461AFP680

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FAB38461AFP680-100UG
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 669604

View all GRB2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GRB2 (SH2 Domain)

Specificity

Detects human GRB2 (SH2 Domain) in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GRAP2 (SH2 domain; aa 58-149), rhGRB7 (aa 130-274), or rhGRB14 (SH2 domain; aa 439-535) is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GRB2

GRB2 (growth factor receptor-bound 2; also Ash) is a ubiquitously-expressed, 27 kDa member of the GRB2/sem-5 family of adaptor molecules.  It serves as a linker for many intracellular proteins.  Upon BCR ligation, LAB is phosphorylated, binds to GRB2, which subsequently recruits signaling factors.  Upon InsR ligation, GRB2 binds to phosphorylated IRS-1, and serves as a linker to ras-activation.  Human GRB2 is 217 amino acids (aa) in length.  It contains one N-terminal SH3 domain (aa 3-54), a central SH2 domain (aa 60-152), and a C-terminal SH3 domain (aa 160-212).  SH2 domains bind phosphotyrosine motifs; SH3 domains bind proline-rich regions.  There is one splice form that shows a deletion of aa 60-100.  This removes the SH2 domain and initiates apoptosis.  Human GRB2 is over 99% aa identical to mouse and dog GRB2.

Long Name

Growth Factor Receptor-Bound Protein 2

Alternate Names

ASH, EGFRBP-GRB2, Grb3-3

Entrez Gene IDs

2885 (Human); 14784 (Mouse); 81504 (Rat)

Gene Symbol

GRB2

UniProt

P62993

Additional GRB2 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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