Human GATA-4 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2606
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Transgenic Mouse
Applications
Validated:
Western Blot, Immunocytochemistry
Cited:
Immunohistochemistry, Immunocytochemistry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GATA-4
Met27-Phe211
Accession # P43694
Met27-Phe211
Accession # P43694
Specificity
Detects human GATA‑4 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human GATA-1, -2, -3, -5, and -6 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human GATA-4 Biotinylated Antibody
GATA‑4 in iBJ6 iPS Cell Line.
GATA-4 was detected in immersion fixed iBJ6, an induced pluripotent stem cell line, differentiated to endoderm using Goat Anti-Human GATA-4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2606) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human GATA-4 Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed endoderm differentiated BG01V human embryonic stem cells and iBJ6, an induced pluripotent stem (iPS) cell line, differentiated to endoderm
Sample: Immersion fixed endoderm differentiated BG01V human embryonic stem cells and iBJ6, an induced pluripotent stem (iPS) cell line, differentiated to endoderm
Western Blot
0.1 µg/mL
Sample: Recombinant Human GATA-4
Sample: Recombinant Human GATA-4
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-4
Additional GATA-4 Products
Product Documents for Human GATA-4 Biotinylated Antibody
Product Specific Notices for Human GATA-4 Biotinylated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...