Detects human gamma-Synuclein in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human beta-Synuclein is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human gamma-Synuclein Antibody

Detection of Human gamma-Synuclein by Western Blot.

Western blot shows lysates of human brain tissue. PVDF Membrane was probed with 1 µg/mL of Human gamma-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5745) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for gamma-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.

gamma-Synuclein in Human Cerebellum.

gamma-Synuclein was detected in immersion fixed paraffin-embedded sections of human cerebellum using Human gamma-Synuclein Antigen Affinity Purified Polyclonal Antibody (Catalog # AF5745) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human gamma-Synuclein Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human cerebellum

Western Blot

1 µg/mL
Sample: Human brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gamma-Synuclein

gamma-Synuclein (SNCG), also called persyn or synoretin, is a 17 kDa, 127 amino acid (aa) cytoplasmic phosphoprotein. It is a member of a family of small, highly conserved synuclein proteins localized predominantly in presynaptic nerve terminals in the brain. SNCG and other synucleins are implicated in neurodegenerative diseases including Parkinson's disease. SNCG expression is elevated in many cancers and is implicated in tumor progression, invasion and proliferation. Within the region used as an immunogen, human SNCG shares 82% and 78% aa identity with mouse and rat SNCG, respectively.

Alternate Names

BCSG1, PRSN, SNCG, Synoretin, Synuclein-gamma

Entrez Gene IDs

6623 (Human)

Gene Symbol

SNCG

UniProt

O76070

Additional gamma-Synuclein Products

Product Documents for Human gamma-Synuclein Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human gamma-Synuclein Antibody

For research use only

Citations
Reviews

Human gamma-Synuclein Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5745

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all gamma-Synuclein Antibodies »

Product Specifications

Immunogen

Specificity