Detects human FOLR2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FOLR1, 3, 4, or recombinant mouse FOLR2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human FOLR2 Antibody

FOLR2 in Human Neutrophils.

FOLR2 was detected in immersion fixed human neutrophils using Mouse Anti-Human FOLR2 Monoclonal Antibody (Catalog # MAB5697) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human FOLR2 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human neutrophils

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR2

Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa glycoprotein that is anchored to the membrane via glycosyl phosphatidylinositol. It mediates the cellular uptake of dietary folates that are required for key steps in amino acid metabolism. FOLR2 is predominantly expressed in placenta, myeloid cells, and some CD34⁺ hematopoietic progenitor cells. It is upregulated on some cancer cells and at sites of chronic inflammation. Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2.

Long Name

Folate Receptor 2

Alternate Names

beta-HFR, FR-beta, FR-P3

Entrez Gene IDs

2350 (Human); 14276 (Mouse); 293154 (Rat)

Gene Symbol

FOLR2

UniProt

P14207 (Human)

Additional FOLR2 Products

Product Documents for Human FOLR2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FOLR2 Antibody

For research use only

Citations
Reviews

Human FOLR2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5697

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all FOLR2 Antibodies »

Product Specifications

Immunogen

Specificity