Detects human FOLR2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FOLR1 and rhFOLR3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human FOLR2 Alexa Fluor™ Plus 680-conjugated Antibody

Application

Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Background: FOLR2

Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3, 4). Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells (4‑6). It is upregulated on myeloid leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 5, 7). It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (8‑10). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (11, 12).

References

Fowler, B. et al. (2001) Kidney Int. 59:S-221.
Kelemen, L.E. (2006) Int. J. Cancer 119:243.
Shen, F. et al. (1994) Biochemistry 33:1209.
Ratnam, M. et al. (1989) Biochemistry 28:8249.
Ross, J.F. et al. (1999) Cancer 85:348.
Reddy, J.A. et al. (1999) Blood 93:3940.
Ross, J.F. et al. (1994) Cancer 73:2432.
Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609.
van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12.
Nagai, T. et al. (2009) Cancer Immunol. Immunother. Feb 24 epub.
Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.

Long Name

Folate Receptor 2

Alternate Names

beta-HFR, FR-beta, FR-P3

Entrez Gene IDs

2350 (Human); 14276 (Mouse); 293154 (Rat)

Gene Symbol

FOLR2

UniProt

P14207 (Human)

Additional FOLR2 Products

Product Documents

Product Datasheets

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COA

SDS

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Product Specific Notices for Human FOLR2 Alexa Fluor™ Plus 680-conjugated Antibody

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Human FOLR2 Alexa Fluor™ Plus 680-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5697AFP680

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