Detects human FGF R1 in direct ELISAs and Western blots. Recognizes all isoforms of recombinant human (rh) FGF R1 and shows10-25% cross-reactivity with various isoforms of rhFGF R2 and rhFGF R3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human FGFR1 Antibody

Detection of Human FGFR1 by Western Blot

Western blot confirmation of marker proteins. Equal quantities of protein from media supernatant from cells cultured as described in Fig 1 were run on Western blot and probed with antibodies for an E8-enriched marker protein (CHGA, NID1, SEMA3 or NPTX3) and an E6-enriched marker protein (COCH, FGFR1, Follistatin or OLFML3). Membranes were imaged using LICOR Odyssey system. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38875182), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human FGFR1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human FGF R1 alpha (IIIb) Fc Chimera (Catalog # 655-FR)
Recombinant Human FGF R1 alpha (IIIc) Fc Chimera (Catalog # 658-FR)
Recombinant Human FGF R1 beta (IIIb) Fc Chimera (Catalog # 765-FR)
Recombinant Human FGF R1 beta (IIIc) Fc Chimera (Catalog # 661-FR)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR1

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Entrez Gene IDs

2260 (Human); 14182 (Mouse)

Gene Symbol

FGFR1

Additional FGFR1 Products

Product Documents for Human FGFR1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FGFR1 Antibody

For research use only

Citations
Reviews

Human FGFR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB658

Key Product Details

Species Reactivity

Validated:

Cited:

Applications