Human ESC Marker Antibody Pack

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42825

Novus Biologicals, part of Bio-Techne
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NBP1-42825
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Key Product Details

Species

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Kit Type

Kit

Product Summary for Human ESC Marker Antibody Pack

This pack contains 1 vial each of: NB100-730 (0.1 mL), NB100-1831 (0.1 mL), NB100-1833 (0.1 mL), NB100-2379 (0.1 mL), NB100-588 (0.1 mL), and NB100-1832 (0.1 mL).

Product Specifications

Specificity

NB100-588 is specific for mouse Nanog protein. NB110-56937:A synthetic peptide corresponding to residues in the 5th cadherin domain of human E-cadherin was used. NB100-1832 Rat Monoclonal to SSEA3 (MC-631) - Embryonic Stem Cell Marker NB100-730 recognizes antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope. NB100-1833 is specific to TRA-1-81 - Embryonic Stem Cell Marker

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for Human ESC Marker Antibody Pack

Live Imaging Microscopy: Human ESC Marker Antibody Pack [NBP1-42825]

Live Imaging Microscopy: Human ESC Marker Antibody Pack [NBP1-42825]

Live Imaging Microscopy: Human ESC Marker Antibody Pack [NBP1-42825] - Pluripotent Li-iPSCs colonies derived from reprogramming of PLCs. iPSC colonies (encircled by dashed line) were positive for the surface pluripotency marker TRA-1-60 (red) during live staining. TRA-1-60 staining (red) is shown before (C) and afteroverlaying onto the corresponding phase contrast image (D). Scale bars = 250um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0221762) licensed under a CC-BY license. TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730]
Immunocytochemistry/ Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825]

Immunocytochemistry/ Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825]

Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # NB100-1833) at 10 ug/mL for 3 hours at room temperature. Cells were stained using an Anti-Mouse IgM Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. TRA-1-81 Antibody (TRA-1-81) [NB100-1833]
Immunocytochemistry/ Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825]

Immunocytochemistry/ Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825]

Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - Analysis of human embryonic stem cells stained with SSEA-3 antibody detected with Alexa Fluor 488 anti-rat IgM secondary antibody. SSEA-3 Antibody (MC-631) [NB100-1832]
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Formulation, Preparation, and Storage

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage is content dependent.

Background: Human ESC Marker

The Human Embryonic Stem Cell Marker Panel is designed to analyze the undifferentiation/differentiation status of human ES cells by analyzing hESC marker expressions. The panel contains the antibodies to well-characterized human embryonic stem cell markers such as Nanog, Oct-3/4, SSEA-1, SSEA-3. and Anti-E-Cadherin. It is useful to identify as well as validate newly isolated or cultured human embryonic stem cell (hESC).

Additional Human ESC Marker Products

Product Documents for Human ESC Marker Antibody Pack

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human ESC Marker Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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