NIH-3T3 mouse embryonic fibroblast cell line transfected with (A) human DC-SIGN and (B) human DC-SIGNR was stained with Mouse Anti-Human DC-SIGN+DC-SIGNR PE-conjugated Monoclonal Antibody (Catalog # FAB1621P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: NIH‑3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGN and human DC-SIGNR

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: DC-SIGN+DC-SIGNR

DC-SIGN (Dendritic Cell-Specific ICAM-3 Grabbing Non-integrin) has been shown to play an important role in regulating Dendritic Cell (DC) and T cell interactions, including antigen presentation to T cells and enhancement of transinfection of CD4+ T cells by HIV-1 (1, 2). Efforts to identify additional type II membrane proteins resulted in the isolation of a molecule related in sequence to DC-SIGN known as DC-SIGNR (DC-SIGN Related) (3, 4). DC-SIGNR shares 73 - 80% amino acid homology with DC-SIGN and is located on human chromosome 19p13.3. Its structure is similar to DC-SIGN and therefore binds mannose residues in a calcium dependent fashion, including ICAM-3 and HIV-1 gp120 (5). DC-SIGNR, also known as L-SIGN (Liver/Lymph node-Specific ICAM-3-Grabbing Non-integrin) and DC‑SIGNR, is polymorphic since allelic variations of the exon 4 encoded sequence have been isolated (5). This is further supported by a study demonstrating the ability to isolate a large repertoire of DC-SIGNR transcripts largely the result of alternative splicing of the 7 coding exons (6). L-SIGN/DC-SIGNR is primarily transcribed in the liver and lymph nodes but not in monocyte derived DC (5). Expression of L-SIGN/DC-SIGNR is restricted to endothelial cells derived from liver sinusoids, lymph node sinuses and capillaries (7) although variable expression in placenta and some monocytic cell lines has also been reported, including both membrane and soluble isoforms of the protein (6). Expression of DC-SIGN is induced during the in vitro generation of DC from either monocytes or bone marrow progenitors, with maximal surface expression at day 7 of culture (1). Immature DC in the skin and mature DC in the tonsil have been demonstrated to express DC‑SIGN (8). Analysis of various tissues and cell lines suggests that DC-SIGN expression is restricted to DC (1) although a more recent report finds evidence of expression in placenta, resting monocytes and monocytic cell lines (6). This discrepancy may be partially related to the multiple isoforms of DC-SIGN transcripts, including both membrane and soluble forms, as well as exon splice variants reported in the latter study (6).

References

Geijtenbeek, T.B.H. et al. (2000) Cell 100:575.
Geijtenbeek, T.B.H. et al. (2000) Cell 100:587.
Yokoyama-Kobayashi, M.T. et al. (1999) Gene 228:161.
Soilleux, E.J. et al. (2000) J. Immunol. 165:2937.
Bashirova, A.A. et al. (2001) J. Exp. Med. 193:671.
Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196..
Pohlman, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
Geijtenbeek, T.B.H. et al. (2000) Nature Immunol. 1:353.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin

Alternate Names

DCSIGN+DCSIGNR

Entrez Gene IDs

30835 (Human)

Gene Symbol

CD209

UniProt

Q9H2X3

Additional DC-SIGN+DC-SIGNR Products

Product Documents for Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

For research use only

Citations
Reviews

Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1621P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all DC-SIGN+DC-SIGNR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

Detection of DC‑SIGN+DC‑SIGNR in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN and DC-SIGNR by Flow Cytometry.

Applications for Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: DC-SIGN+DC-SIGNR

References

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional DC-SIGN+DC-SIGNR Products

Product Documents for Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Human DC-SIGN+DC-SIGNR PE-conjugated Antibody