Detects human CXCL7/NAP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) ENA-70, rhENA-74, rhENA-78, rhGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhIL-8, rhIP-10, rhMIG, rhSDF‑1 alpha, and rhSDF‑1 beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CXCL7/NAP‑2 Biotinylated Antibody

Human CXCL7 / NAP-2 ELISA Standard Curve

Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP) was serially diluted and captured by Mouse Anti-Human CXCL7/NAP-2 Monoclonal Antibody (Catalog # MAB393) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL7/NAP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF393) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CXCL7/NAP‑2 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP)

Human CXCL7/NAP-2 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Human CXCL7/NAP-2 Antibody (Catalog # MAB393)
Standard: Recombinant Human CXCL7/NAP-2 Protein (Catalog # 393-NP)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL7/NAP-2

Neutrophil Activating Peptide 2 (NAP-2), Connective Tissue Activating Protein III (CTAP‑III) and beta-thrombogulin ( beta‑TG), are proteolytically processed carboxyl-terminal fragments of platelet basic protein (PBP) which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR2 and to chemoattract and activate neutrophils. Although CTAP-III, beta-TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. It has been shown that the additional amino-terminal residues of CTAP-III masks the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.

References

Schall, T. (1994) The Cytokine Handbook, 2nd edition, A. Thomson, ed. Academic Press, New York, p. 419.
Malkowski, M.G. et al. (1997) J. Mol. Biol. 266:367.

Alternate Names

CTAP III, NAP-2, NAP2, PPBP

Entrez Gene IDs

5473 (Human)

Gene Symbol

PPBP

UniProt

P02775

Additional CXCL7/NAP-2 Products

Product Documents for Human CXCL7/NAP‑2 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human CXCL7/NAP‑2 Biotinylated Antibody

For research use only

Citations
Reviews

Human CXCL7/NAP‑2 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF393

Key Product Details

Species Reactivity

Validated:

Cited:

Applications