HEK293 human embryonic kidney cell line transfected with human Connexin 43/GJA1 and eGFP was stained with and either (A) Mouse Anti-Human Connexin 43/GJA1 APC-conjugated Monoclonal Antibody (Catalog # FAB7737A) or (B) Mouse IgG_2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Connexin 43/GJA1 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human Connexin 43/GJA1 and eGFP

Reviewed Applications

Read 1 review rated 4 using FAB7737A in the following applications:

Flow Cytometry (1 Review)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Connexin 43/GJA1

Connexin43 (Cx43; also gap junction alpha-1 protein) is a 41 - 44 kDa member of the connexin family, alpha-type subfamily, of transmembrane proteins. It is the most common type of connexin in cardiac muscle cells, and also occurs in hepatocytes, astrocytes and ovary granulosa cells. It is a 4-transmembrane protein 382 amino acids (aa) in length that contains two cytoplasmic tails. One is 12 aa in length at the N-terminus and the second is 151 aa in length at the C-terminus. Human and rat Cx43 are 98% aa identical over the entire length of the molecule. Connexins form gap junctions (GJs) which are intercellular channels between cells. Each adjacent cell contributes to a functional channel. The fundamental unit is a “connexon”, or hemi-channel, which is composed of six connexins in a sliding subunit hexamer configuration. The hexamer may be either homomeric or heteromeric. The connexon arrangement provides for the opening and closing of an intersubunit space (or pore) that allows diffusion of molecules 1 kDa or less. It is suggested that the extended C-terminus of each connexin may interact with multiple docking proteins and serve as a plug during closure. Connexons on adjacent cells interact via their extracellular loops to form a GJ channel. When open, these channels allow for the transit of small hydrophilic molecules such as ATP, glucose, IP3 and Ca++. Connexons (or hemi-channels) that do not interact with adjacent cells are also suggested to allow for small molecule transit under unusual circumstances. Cx43 activity is regulated by phosphorylation. The exact effects, however, may be context-specific. In one case, PKC phosphorylation of S368 has been found to decrease GJ channel permeability to select small molecular weight solutes. Alternatively, PKA has been suggested to be the mediator of Cx43 phosphorylation of S365, S368, S369 and S373. In this case, phosphorylation promotes channel activity.

References

Sohl, G. and K. Willecke (2004) Cardiovasc. Res. 62:228.
Willecke, K. et al. (2002) Biol. Chem. 383:725.
Giepmans, B.N.G. (2004) Cardiovasc. Res. 62:233.
Vinken, M. et al. (2006) Cell. Signal. 18:592.
Stout, C. et al. (2004) Curr. Opin. Cell Biol. 16:507.
Bao, X. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C647.
Yogo, K. et al. (2006) J. Reprod. Dev. 52:321.

Alternate Names

CX43, GJA1, GJAL, ODDD, ODOD, SDTY3

Entrez Gene IDs

2697 (Human)

Gene Symbol

GJA1

UniProt

P17302

Additional Connexin 43/GJA1 Products

Product Documents for Human Connexin 43/GJA1 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Connexin 43/GJA1 APC-conjugated Antibody

For research use only

Citations
Reviews

Human Connexin 43/GJA1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7737A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Connexin 43/GJA1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Connexin 43/GJA1 APC-conjugated Antibody

Detection of Connexin 43/GJA1 in HEK293 Human Cell Line Transfected with Human Connexin 43/GJA1 and eGFP by Flow Cytometry.

Applications for Human Connexin 43/GJA1 APC-conjugated Antibody

Flow Cytometry

Reviewed Applications

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: Connexin 43/GJA1

References

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Connexin 43/GJA1 Products

Product Documents for Human Connexin 43/GJA1 APC-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Human Connexin 43/GJA1 APC-conjugated Antibody