Human CKAP4/p63 Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB11600R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Mouse IgG Clone # 1086526
Product Specifications
Immunogen
Mouse myeloma cell line, NS0-derived human CKAP-4
His128-Val602
Accession # Q07065
His128-Val602
Accession # Q07065
Specificity
Detects recombinant human CKAP4 in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Applications for Human CKAP4/p63 Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: CKAP4/p63
CKAP4 (Cytoskeleton-associated protein 4; also CLIMP63 and p63) is a 63-64 kDa molecule that belongs to no known protein family. It is found intracellularly, and on the plasma membrane of select cells such as vascular smooth muscle and Type II Greater alveolar lung cells. CKAP4 has a bimodal distribution. First, it is embedded in the membrane of a compartment that links the ER with the Golgi apparatus. This localization is dependent upon its ability to form homooligomers, and its presence serves to anchor microtubules and direct the formation of tubular ER. Second, it is embedded in the plasma membrane and serves as a receptor for SP-A/surfactant protein-A (in lung) and tPA (in vessels). Human CKAP4 is a 602 amino acid (aa) type II transmembrane nonglycosylated protein. It contains a 106 aa N-terminal cytoplasmic region plus a 475 aa C-terminal luminal domain (aa 128-602). The luminal domain contains three coiled-coil regions (aa 130-214; 256-460; 533-602) plus three utilized phosphorylation sites. CKAP4 undergoes reversible palmitoylation. There are two potential isoform variants. One contains an alternative start at Met269, while another shows a deletion of aa 258-435. Over aa 128-602, human CKAP4 shares 82% aa sequence identity with mouse CKAP4.
Long Name
Cytoskeleton-associated Protein-4/Climp-63
Alternate Names
CLIMP-63, ERGIC-63, p63
Gene Symbol
CKAP4
UniProt
Additional CKAP4/p63 Products
Product Specific Notices for Human CKAP4/p63 Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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