PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Chondrolectin Monoclonal Antibody (Catalog # MAB2576, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Chondrolectin in PC‑3 Human Cell Line.

Chondrolectin was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human Chondrolectin Monoclonal Antibody (Catalog # MAB2576) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Chondrolectin Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: PC-3 human prostate cancer cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed PC-3 human prostate cancer cell line

Western Blot

1 µg/mL
Sample: Recombinant Human Chondrolectin

Reviewed Applications

Read 1 review rated 5 using MAB2576 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chondrolectin

Chondrolectin (CHODL) is a 36 kDa type I transmembrane protein containing a C-type lectin carbohydrate recognition domain. Multiple isoforms of human CHODL are generated as a result of alternative promoter usage and/or alternative splicing. Some isoforms are differentially expressed during T cell development. Human and mouse CHODL share 94% amino acid sequence identity in their extracellular region.

Alternate Names

C21orf68, CHODL, MT75, PRED12

Entrez Gene IDs

140578 (Human); 246048 (Mouse)

Gene Symbol

CHODL

UniProt

Q9H9P2

Additional Chondrolectin Products

Product Documents for Human Chondrolectin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Chondrolectin Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Chondrolectin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2576

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Chondrolectin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Chondrolectin Antibody

Detection of Chondrolectin in PC-3 Human Cell Line by Flow Cytometry.