THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CEACAM-4 Monoclonal Antibody (Catalog # MAB7845, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human CEACAM-4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: THP‑1 human acute monocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-4

CEACAMs are intercellular adhesion molecules that form Ca²⁺-dependent homo- and heterotypic interactions, and typically serve as pathogen receptors. All CEACAMs are heavily glycosylated and contain one N-terminal V-type Ig-like domain. CEACAMs also exhibit variability in their structure by containing transmembrane domains (#CEACAM-1, 3 & 4), GPI-linked domains (#5, 6, 7, & 8), or CD66 designations (#1, 3, 5, 6, & 7).

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 4

Alternate Names

CEACAM4, CGM7

Entrez Gene IDs

1089 (Human)

Gene Symbol

CEACAM4

UniProt

O75871

Additional CEACAM-4 Products

Product Documents for Human CEACAM-4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CEACAM-4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CEACAM-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7845

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CEACAM-4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CEACAM-4 Antibody

Detection of CEACAM‑4 in THP‑1 Human Cell Line by Flow Cytometry.