Human CD53 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4624A

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 425514

View all CD53 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CD53
Met1-Leu219
Accession # P19397

Specificity

Detects human CD53. Stains human CD53 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD53 APC-conjugated Antibody

Detection of CD53 antibody in Human Peripheral Blood Lymphocytes antibody by Flow Cytometry.

Detection of CD53 in Human Peripheral Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD53 APC-conjugated Monoclonal Antibody (Catalog # FAB4624A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD53 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD53

CD53, also known as TSPAN25, is a 35 kDa‑40 kDa cell surface and endosomal membrane glycoprotein that belongs to the 4 TM tetraspanin superfamily of molecules. CD53 is widely expressed on hematopoietic cells, including eosinophils, neutrophils, mast cells, CD16+ proinflammatory monocytes, NK cells T cells, macrophages, splenic B cells and immature dendritic cells. Ligation of CD53 promotes cell proliferation and survival, as well as homotypic cell‑cell adhesion. On the cell membrane, CD53 associates with other tetraspanins, MHC class II molecules, and alpha3 and alpha4 integrins. Human CD53 shares 71% aa sequence identity with mouse and CD53 in the extracellular domains.

Alternate Names

CD53, MOX44, TSPAN25

Entrez Gene IDs

963 (Human); 12508 (Mouse); 24251 (Rat)

Gene Symbol

CD53

UniProt

P19397

Additional CD53 Products

Product Documents for Human CD53 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD53 APC-conjugated Antibody

For research use only

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