Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD36/SR-B3 Mono-clonal Antibody (Catalog # MAB19552) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD36/SR-B3 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CD36/SR‑B3 in Human Heart.

CD36/SR-B3 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human CD36/SR-B3 Monoclonal Antibody (Catalog # MAB19552) at 5 µg/mL for 1 hour at room temperature followed by incubation with Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained with DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human CD36/SR-B3 by Simple Western^TM.

Simple Western lane view shows lysates of human platelets, loaded at 0.5 mg/mL. A specific band was detected for CD36/SR‑B3 at approximately 138 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CD36/SR‑B3 Monoclonal Antibody (Catalog # MAB19552) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Applications for Human CD36/SR-B3 Antibody

Application

Recommended Usage

Immunohistochemistry

5 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Simple Western

10-50 µg/mL
Sample: Human platelets and Human adipose tissue

Western Blot

1 µg/mL
Sample: Human placenta tissue and human platelets

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD36/SR-B3

CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta‑amyloid fibrils (fA beta), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.

References

Febbraio, M. et al. (2001) J. Clin. Invest. 108:785.
Khoury, J. et al. (2003) J. Exp. Med. 197:1657.
Husemann, J. et al. (2002) Glia 40:195.
Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63.
Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.

Long Name

Scavenger Receptor Class B, Member 3

Alternate Names

CD36, Collagen R, FAT, GPIIIb, GPIV, SCARB3, SR-B3, SRB3, Thrombospondin R

Entrez Gene IDs

948 (Human); 12491 (Mouse)

Gene Symbol

CD36

UniProt

P16671

Additional CD36/SR-B3 Products

Product Documents for Human CD36/SR-B3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD36/SR-B3 Antibody

For research use only

Citations
Reviews

Human CD36/SR-B3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB19552

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD36/SR-B3 Antibody

Detection of Human CD36/SR‑B3 by Western Blot.