Human CD300a/LMIR1 Alexa Fluor™ Plus 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2640AFP594

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AF2640AFP594-100UG
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Polyclonal Goat IgG

View all CD300a/LMIR1 Antibodies »

Product Specifications

Specificity

Detects human CD300a/LMIR1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) LMIR2, rhLMIR3, rhLMIR5, and rhLMIR6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

ELISA

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Background: CD300a/LMIR1

CD300a, also known as LMIR1 (in rodents), CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Human LMIR1 consists of a 163 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 98 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2). Alternate splicing may generate additional isoforms that either lack the Ig-like domain or contain only the cytoplasmic domain. Within the ECD, human LMIR1 shares 40% and 43% aa sequence identity with mouse and rat LMIR1, respectively. In human, LMIR1 is expressed on peripheral blood eosinophils, mast cells, neutrophils, plasmacytoid dendritic cells, and various T cell subsets (3‑7). Antibody crosslinking of LMIR1 induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Crosslinking of LMIR1 to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (5, 10‑12). LMIR1 crosslinking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 11, 12).

References

  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.
  3. Munitz, A. et al. (2006) Blood 107:1996.
  4. Bachelet, I. et al. (2005) J. Immunol. 175:7989.
  5. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.
  6. Ju, X. et al. (2008) Blood 112:1184.
  7. Clark, G.J. et al. (2007) J. Leukoc. Biol. 82:1126.
  8. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.
  9. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
  10. Bachelet, I. et al. (2008) J. Immunol. 180:6064.
  11. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.
  12. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.

Alternate Names

CD300a, CLM8, CMRF-35H, IGSF12, IRC1, IRC2, IRp60, LMIR1, MAIR-I, Mcipr1, NKRL, Pigr4

Entrez Gene IDs

11314 (Human); 217303 (Mouse)

Gene Symbol

CD300A

UniProt

Q9UGN4

Additional CD300a/LMIR1 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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