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Human CD28 Antibody Best Seller
Catalog # MAB342 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Agonist Activity, Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 37407
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Product Summary for Human CD28 Antibody
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD28
Asn19-Pro152
Accession # P10747
Asn19-Pro152
Accession # P10747
Specificity
Detects human CD28 in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) CD28, rhCTLA4, or rmCTLA4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD28 Antibody
Human CD28 Antibody Enhances IL-2 Secretion in Jurkat Cells.
Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 µM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (D2050). The ED50 for this effect is typically 0.2-0.6 µg/mL.
Detection of CD28 in U266 human myeloma cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative).
CD28 was detected in immersion fixed U266 human myeloma cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative) using Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human CD28 Antibody
Application
Recommended Usage
Agonist Activity
0.2-0.6 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed U266 human myeloma cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative)
Sample: Immersion fixed U266 human myeloma cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative)
Western Blot
1 µg/mL
Sample: Recombinant Human CD28 Fc Chimera (Catalog # 342-CD)
under non-reducing conditions only
Sample: Recombinant Human CD28 Fc Chimera (Catalog # 342-CD)
under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB342 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from ascites
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD28
CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig
V‑like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is up-regulated rapidly following T cell activation and CD28 ligation. Cell surface expression of mouse CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the upregulation of Bcl-xL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.
Product Documents for Human CD28 Antibody
Product Specific Notices for Human CD28 Antibody
For research use only
Citations for Human CD28 Antibody (38)
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Citations are publications that use Bio-Techne products. Selected citations for Human CD28 Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryA Lanna et al. (2022-09-15), An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory Nature Cell Biology, 2022-00(0).
PMID: 36109671 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayL Yang et al. (2022-03-26), Overexpression of ascitic interleukin-35 induces CD8+ T cell exhaustion in liver cirrhotic patients with spontaneous bacterial peritonitis International immunopharmacology, 2022-0108(0):108729.
PMID: 35349961 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayGuo J et al. (2022-02-01), Eradicating tumor in a recurrent cervical cancer patient with autologous tumor-infiltrating lymphocytes and a modified lymphodepleting regimen. J Immunother Cancer, 2022-010(2).
PMID: 35177415 -
Species: Human
Sample Types: Whole Cells
Applications: ICCMG Jeong et al. (2021-02-19), Analysis of transient membrane protein interactions by single-molecule diffusional mobility shift assay Experimental & Molecular Medicine, 2021-00(0).
PMID: 33603128 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayX Zhuang et al. (2020-10-02), CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth JCI Insight, 2020-15(19).
PMID: 33004686 -
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay,T Cell ActivationJ Huetter et al. (2020-04-20), Characterization of BAY 1905254, an Immune Checkpoint Inhibitor Targeting the Immunoglobulin-Like Domain Containing Receptor 2 (ILDR2) Cancer Immunol Res, 2020-08(7):895-911.
PMID: 32312711 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryZ Hu et al. (2020-03-26), PCC0208025 (BMS202), a small molecule inhibitor of PD-L1, produces an antitumor effect in B16-F10 melanoma-bearing mice PLoS ONE, 2020-015(3):e0228339.
PMID: 32214351 -
Species: Human
Sample Types: Whole Tissue
Applications: Tissue CultureML Tiirikaine et al. (2019-12-27), Ex vivo culture of lesional psoriasis skin for pharmacological testing J. Dermatol. Sci., 2019-10(0).
PMID: 31948839 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayY Lu et al. (2019-10-14), 4D electron microscopy of T cell activation Proc. Natl. Acad. Sci. U.S.A., 2019-1116(44):22014-22019.
PMID: 31611413 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayJC Chamcheu et al. (2019-09-15), Fisetin, a 3,7,3',4'-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models Cells, 2019-08(9).
PMID: 31540162 -
Species: Human
Sample Types: Whole Cells
Applications: StimulationNB Zhou et al. (2019-08-16), Effect of morphine and a low dose of ketamine on the T cells of patients with refractory cancer pain in vitro Oncol Lett, 2019-018(4):4230-4236.
PMID: 31516618 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayF Bensch et al. (2018-11-26), 89Zr-atezolizumab imaging as a non-invasive approach to assess clinical response to PD-L1 blockade in cancer Nat. Med., 2018-10(0).
PMID: 30478423 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayV Giacomet et al. (2018-02-14), Humoral and cell-mediated immune responses after a booster dose of HBV vaccine in HIV-infected children, adolescents and young adults PLoS ONE, 2018-013(2):e0192638.
PMID: 29444185 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryC Caër et al. (2017-06-07), Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue Sci Rep, 2017-07(1):3000.
PMID: 28592801 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayS Nakachi et al. (2017-05-16), Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis Arthritis Res. Ther., 2017-019(1):97.
PMID: 28511719 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayC Tao et al. (2017-02-15), ??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function Mol Med Rep, 2017-00(0).
PMID: 28259946 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: PolarizationMoore C et al. (2014-12-10), Direct and indirect effects of immune and central nervous system-resident cells on human oligodendrocyte progenitor cell differentiation. J Immunol, 2014-1194(2):761-72.
PMID: 25505283 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationVan Belle T et al. (2014-10-03), 1,25-Dihydroxyvitamin D3 and its analog TX527 promote a stable regulatory T cell phenotype in T cells from type 1 diabetes patients. PLoS ONE, 2014-19(10):e109194.
PMID: 25279717 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayZhu L et al. (2014-06-27), Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation. J Thromb Haemost, 2014-012(7):1156-65.
PMID: 24833264 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayFoley M et al. (2014-02-13), High avidity CD8+ T cells efficiently eliminate motile HIV-infected targets and execute a locally focused program of anti-viral function. PLoS ONE, 2014-09(2):e87873.
PMID: 24551068 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationDalmas E et al. (2014-02-11), T cell-derived IL-22 amplifies IL-1beta-driven inflammation in human adipose tissue: relevance to obesity and type 2 diabetes. Diabetes, 2014-063(6):1966-77.
PMID: 24520123 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayRenand A et al. (2013-12-30), Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs. PLoS ONE, 2013-18(12):e85589.
PMID: 24386482 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGironi et al. (2013-01-10), A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis. Immun Ageing, 2013-010(1):1.
PMID: 23305498 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWolk K et al. (2009-12-01), The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis. Eur. J. Immunol., 2009-139(12):3570-81.
PMID: 19830738 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayHirosako S et al. (2008-11-26), Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells. Clin. Exp. Immunol., 2008-1155(2):266-74.
PMID: 19040600 -
Species: Human
Sample Types: Whole Cells
Applications: ICCLazer G et al. (2007-08-08), The association of Sam68 with Vav1 contributes to tumorigenesis. Cell. Signal., 2007-019(12):2479-86.
PMID: 17855053 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayCelis E et al. (2007-07-01), Overlapping human leukocyte antigen class I/II binding peptide vaccine for the treatment of patients with stage IV melanoma: evidence of systemic immune dysfunction. Cancer, 2007-0110(1):203-14.
PMID: 17541944 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayBayry J et al. (2007-04-01), Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells. J. Immunol., 2007-0178(7):4184-93.
PMID: 17371975 -
Species: Human
Sample Types: Plasma
Applications: ELISA DevelopmentSakthivel P et al. (2007-04-01), The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. J. Intern. Med., 2007-0261(4):399-407.
PMID: 17391115 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayPiconi S et al. (2007-02-01), Effects of specific immunotherapy on the B7 family of costimulatory molecules in allergic inflammation. J. Immunol., 2007-0178(3):1931-7.
PMID: 17237444 -
Species: Human
Sample Types: Whole Cells
Applications: Binding AssaySrinivasan M et al. (2007-02-01), Suppression of immune responses in collagen-induced arthritis by a rationally designed CD80-binding peptide agent. Arthritis Rheum., 2007-056(2):498-508.
PMID: 17265485 -
Species: Human
Sample Types: Recombinant Protein
Applications: Western BlotNeuber K et al. (2006-11-01), Autoantibodies against CD28 are associated with atopic diseases. Clin. Exp. Immunol., 2006-1146(2):262-9.
PMID: 17034578 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayMamoni RL et al. (2006-10-11), Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis. Cytokine, 2006-135(3):207-16.
PMID: 17045486 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayOgilvie RL et al. (2005-01-15), Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay. J. Immunol., 2005-0174(2):953-61.
PMID: 15634918 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWolk K et al. (2004-08-01), IL-22 increases the innate immunity of tissues. Immunity, 2004-021(2):241-54.
PMID: 15308104 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWang P et al. (2004-06-01), Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor. Biochem. J., 2004-0380(0):425-33.
PMID: 14992689 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayJanardhan A et al. (2004-01-20), HIV-1 Nef binds the DOCK2-ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis. PLoS Biol., 2004-02(1):E6.
PMID: 14737186 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWolk K et al. (2002-06-01), Cutting edge: immune cells as sources and targets of the IL-10 family members? J. Immunol., 2002-0168(11):5397-402.
PMID: 12023331
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Customer Reviews for Human CD28 Antibody (2)
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Application: Western BlotSample Tested: T cellsSpecies: HumanVerified Customer | Posted 02/10/2022
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Application: Functional AssaySample Tested: Peripheral blood T cellsSpecies: HumanVerified Customer | Posted 02/16/201996-well plates were coated with anti-CD3e -/+ MAB342 and incubated with isolated CD8+ T-cells from human PBMCs. Activation was read through intracellular interferon.
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