Human CD28 Antibody Best Seller
Catalog # MAB342 | R&D Systems, Inc. a Bio-Techne Brand
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Product Summary for Human CD28 Antibody
Immunogen
Asn19-Pro152
Accession # P10747
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human CD28 Antibody
Human CD28 Antibody Enhances IL-2 Secretion in Jurkat Cells.
Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 µM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (D2050). The ED50 for this effect is typically 0.2-0.6 µg/mL.Detection of CD28 in U266 human myeloma cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative).
CD28 was detected in immersion fixed U266 human myeloma cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative) using Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human CD28 Antibody
Agonist Activity
Sample: Jurkat human acute T cell leukemia cell line
Immunocytochemistry
Sample: Immersion fixed U266 human myeloma cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative)
Western Blot
Sample: Recombinant Human CD28 Fc Chimera (Catalog # 342-CD)
under non-reducing conditions only
Reviewed Applications
Read 2 reviews rated 5 using MAB342 in the following applications:
Formulation, Preparation and Storage
Purification
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Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD28
CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig
V‑like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is up-regulated rapidly following T cell activation and CD28 ligation. Cell surface expression of mouse CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the upregulation of Bcl-xL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation.
Product Documents for Human CD28 Antibody
Product Specific Notices for Human CD28 Antibody
For research use only
Citations for Human CD28 Antibody (38)
Citations are publications that use Bio-Techne products. Selected citations for Human CD28 Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryA Lanna et al. (2022-09-15), An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory Nature Cell Biology, 2022-00(0).
PMID: 36109671 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayL Yang et al. (2022-03-26), Overexpression of ascitic interleukin-35 induces CD8+ T cell exhaustion in liver cirrhotic patients with spontaneous bacterial peritonitis International immunopharmacology, 2022-0108(0):108729.
PMID: 35349961 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayGuo J et al. (2022-02-01), Eradicating tumor in a recurrent cervical cancer patient with autologous tumor-infiltrating lymphocytes and a modified lymphodepleting regimen. J Immunother Cancer, 2022-010(2).
PMID: 35177415 -
Species: Human
Sample Types: Whole Cells
Applications: ICCMG Jeong et al. (2021-02-19), Analysis of transient membrane protein interactions by single-molecule diffusional mobility shift assay Experimental & Molecular Medicine, 2021-00(0).
PMID: 33603128 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayX Zhuang et al. (2020-10-02), CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth JCI Insight, 2020-15(19).
PMID: 33004686 -
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay,T Cell ActivationJ Huetter et al. (2020-04-20), Characterization of BAY 1905254, an Immune Checkpoint Inhibitor Targeting the Immunoglobulin-Like Domain Containing Receptor 2 (ILDR2) Cancer Immunol Res, 2020-08(7):895-911.
PMID: 32312711 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryZ Hu et al. (2020-03-26), PCC0208025 (BMS202), a small molecule inhibitor of PD-L1, produces an antitumor effect in B16-F10 melanoma-bearing mice PLoS ONE, 2020-015(3):e0228339.
PMID: 32214351 -
Species: Human
Sample Types: Whole Tissue
Applications: Tissue CultureML Tiirikaine et al. (2019-12-27), Ex vivo culture of lesional psoriasis skin for pharmacological testing J. Dermatol. Sci., 2019-10(0).
PMID: 31948839 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayY Lu et al. (2019-10-14), 4D electron microscopy of T cell activation Proc. Natl. Acad. Sci. U.S.A., 2019-1116(44):22014-22019.
PMID: 31611413 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayJC Chamcheu et al. (2019-09-15), Fisetin, a 3,7,3',4'-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models Cells, 2019-08(9).
PMID: 31540162 -
Species: Human
Sample Types: Whole Cells
Applications: StimulationNB Zhou et al. (2019-08-16), Effect of morphine and a low dose of ketamine on the T cells of patients with refractory cancer pain in vitro Oncol Lett, 2019-018(4):4230-4236.
PMID: 31516618 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayF Bensch et al. (2018-11-26), 89Zr-atezolizumab imaging as a non-invasive approach to assess clinical response to PD-L1 blockade in cancer Nat. Med., 2018-10(0).
PMID: 30478423 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayV Giacomet et al. (2018-02-14), Humoral and cell-mediated immune responses after a booster dose of HBV vaccine in HIV-infected children, adolescents and young adults PLoS ONE, 2018-013(2):e0192638.
PMID: 29444185 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryC Caër et al. (2017-06-07), Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue Sci Rep, 2017-07(1):3000.
PMID: 28592801 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayS Nakachi et al. (2017-05-16), Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis Arthritis Res. Ther., 2017-019(1):97.
PMID: 28511719 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayC Tao et al. (2017-02-15), ??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function Mol Med Rep, 2017-00(0).
PMID: 28259946 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: PolarizationMoore C et al. (2014-12-10), Direct and indirect effects of immune and central nervous system-resident cells on human oligodendrocyte progenitor cell differentiation. J Immunol, 2014-1194(2):761-72.
PMID: 25505283 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationVan Belle T et al. (2014-10-03), 1,25-Dihydroxyvitamin D3 and its analog TX527 promote a stable regulatory T cell phenotype in T cells from type 1 diabetes patients. PLoS ONE, 2014-19(10):e109194.
PMID: 25279717 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayZhu L et al. (2014-06-27), Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation. J Thromb Haemost, 2014-012(7):1156-65.
PMID: 24833264 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayFoley M et al. (2014-02-13), High avidity CD8+ T cells efficiently eliminate motile HIV-infected targets and execute a locally focused program of anti-viral function. PLoS ONE, 2014-09(2):e87873.
PMID: 24551068 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationDalmas E et al. (2014-02-11), T cell-derived IL-22 amplifies IL-1beta-driven inflammation in human adipose tissue: relevance to obesity and type 2 diabetes. Diabetes, 2014-063(6):1966-77.
PMID: 24520123 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayRenand A et al. (2013-12-30), Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs. PLoS ONE, 2013-18(12):e85589.
PMID: 24386482 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGironi et al. (2013-01-10), A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis. Immun Ageing, 2013-010(1):1.
PMID: 23305498 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWolk K et al. (2009-12-01), The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis. Eur. J. Immunol., 2009-139(12):3570-81.
PMID: 19830738 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayHirosako S et al. (2008-11-26), Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells. Clin. Exp. Immunol., 2008-1155(2):266-74.
PMID: 19040600 -
Species: Human
Sample Types: Whole Cells
Applications: ICCLazer G et al. (2007-08-08), The association of Sam68 with Vav1 contributes to tumorigenesis. Cell. Signal., 2007-019(12):2479-86.
PMID: 17855053 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayCelis E et al. (2007-07-01), Overlapping human leukocyte antigen class I/II binding peptide vaccine for the treatment of patients with stage IV melanoma: evidence of systemic immune dysfunction. Cancer, 2007-0110(1):203-14.
PMID: 17541944 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayBayry J et al. (2007-04-01), Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells. J. Immunol., 2007-0178(7):4184-93.
PMID: 17371975 -
Species: Human
Sample Types: Plasma
Applications: ELISA DevelopmentSakthivel P et al. (2007-04-01), The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. J. Intern. Med., 2007-0261(4):399-407.
PMID: 17391115 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayPiconi S et al. (2007-02-01), Effects of specific immunotherapy on the B7 family of costimulatory molecules in allergic inflammation. J. Immunol., 2007-0178(3):1931-7.
PMID: 17237444 -
Species: Human
Sample Types: Whole Cells
Applications: Binding AssaySrinivasan M et al. (2007-02-01), Suppression of immune responses in collagen-induced arthritis by a rationally designed CD80-binding peptide agent. Arthritis Rheum., 2007-056(2):498-508.
PMID: 17265485 -
Species: Human
Sample Types: Recombinant Protein
Applications: Western BlotNeuber K et al. (2006-11-01), Autoantibodies against CD28 are associated with atopic diseases. Clin. Exp. Immunol., 2006-1146(2):262-9.
PMID: 17034578 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayMamoni RL et al. (2006-10-11), Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis. Cytokine, 2006-135(3):207-16.
PMID: 17045486 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayOgilvie RL et al. (2005-01-15), Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay. J. Immunol., 2005-0174(2):953-61.
PMID: 15634918 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWolk K et al. (2004-08-01), IL-22 increases the innate immunity of tissues. Immunity, 2004-021(2):241-54.
PMID: 15308104 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWang P et al. (2004-06-01), Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor. Biochem. J., 2004-0380(0):425-33.
PMID: 14992689 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayJanardhan A et al. (2004-01-20), HIV-1 Nef binds the DOCK2-ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis. PLoS Biol., 2004-02(1):E6.
PMID: 14737186 -
Species: Human
Sample Types: Whole Cells
Applications: Functional AssayWolk K et al. (2002-06-01), Cutting edge: immune cells as sources and targets of the IL-10 family members? J. Immunol., 2002-0168(11):5397-402.
PMID: 12023331
There are no citations that match your criteria.
Customer Reviews for Human CD28 Antibody (2)
Customer Images
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Application: Western BlotSample Tested: T cellsSpecies: HumanVerified Customer | Posted 02/10/2022
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Application: Functional AssaySample Tested: Peripheral blood T cellsSpecies: HumanVerified Customer | Posted 02/16/201996-well plates were coated with anti-CD3e -/+ MAB342 and incubated with isolated CD8+ T-cells from human PBMCs. Activation was read through intracellular interferon.
There are no reviews that match your criteria.
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