Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Antibody

Detection of CD25/IL-2R alpha in PBMCs treated with 5 ug/mL PHA for 5 days cells by Flow Cytometry.

PBMCs treated with 5 ug/mL PHA for 5 days were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Monoclonal Antibody (Catalog # FAB1020MFV610) or (B) unstained cells. View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Antibody

Application

Recommended Usage

Flow Cytometry

5 µL/10⁶ cells
Sample: PBMCs treated with 5 ug/mL PHA for 5 days

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD25/IL-2R alpha

Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL-2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Gene Symbol

IL2RA

UniProt

P01589

Additional CD25/IL-2R alpha Products

Product Documents

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Antibody

mFluor^TM is a trademark of AAT Bioquest, Inc.

For research use only

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Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1020MFV610

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all CD25/IL-2R alpha Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Antibody

Detection of CD25/IL-2R alpha in PBMCs treated with 5 ug/mL PHA for 5 days cells by Flow Cytometry.

Applications for Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: CD25/IL-2R alpha

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional CD25/IL-2R alpha Products

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Antibody