Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1020MFV610

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
FAB1020MFV610-100
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry

Label

mFluor Violet 610 SE (Excitation = 421 nm, Emission = 613 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 24212

View all CD25/IL-2R alpha Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
Glu22-Cys213
Accession # P01589

Specificity

Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Antibody

Detection of CD25/IL-2R alpha in PBMCs treated with 5 ug/mL PHA for 5 days cells by Flow Cytometry.

PBMCs treated with 5 ug/mL PHA for 5 days were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Monoclonal Antibody (Catalog # FAB1020MFV610) or (B) unstained cells. View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: PBMCs treated with 5 ug/mL PHA for 5 days

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD25/IL-2R alpha

Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL-2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Gene Symbol

IL2RA

UniProt

P01589

Additional CD25/IL-2R alpha Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD25/IL-2R alpha mFluor™ Violet 610-Conjugated Antibody

mFluorTM is a trademark of AAT Bioquest, Inc.

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.