Human CCL4/MIP-1 beta APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC271A
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 24006
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
Ala24-Asn92
Accession # P13236
Ala24-Asn92
Accession # P13236
Specificity
Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, this antibody shows less than 4% cross-reactivity with recombinant human (rh) CCL3/MIP-1 alpha and does not cross-react with recombinant mouse (rm) CCL3/MIP-1 alpha, rmCCL4/MIP-1 beta, rhCXCL8/IL-8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CCL4/MIP-1 beta APC-conjugated Antibody
Detection of CCL4/MIP-1 beta in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCL4/MIP-1 beta APC-conjugated Monoclonal Antibody (Catalog # IC271A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human CCL4/MIP-1 beta APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood monocytes treated with LPS and Monensin were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood monocytes treated with LPS and Monensin were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCL4/MIP-1 beta
Alternate Names
Exodus-3, MIP-1 beta, MIP1 beta
Entrez Gene IDs
Gene Symbol
CCL4
UniProt
Additional CCL4/MIP-1 beta Products
Product Documents for Human CCL4/MIP-1 beta APC-conjugated Antibody
Product Specific Notices for Human CCL4/MIP-1 beta APC-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...