Human CCL3/MIP-1 alpha Antibody

Chemotaxis Induced by CCL3/MIP-1 alpha and Neutralization by Human CCL3/MIP-1 alpha Antibody.

Recombinant Human CCL3/MIP-1a (Catalog # 270-LD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant HumanCCL3/MIP-1a (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL3/MIP-1a Polyclonal Antibody (Catalog # AB-270-NA). The ND₅₀ is typically ≤ 6 µg/mL.

Detection of CCL3/MIP-1 alpha by Immunohistochemistry

Citrulline protected mice against primary CDI with MIP-1 alpha inhibition. (A) Experimental plan of primary CDI in regular mice. Mice were infected with C. difficile VPI10463. Some mice were intraperitoneally injected with 100mg/mouse control IgG (AB-108-C, R&D Systems), 10 mg/mouse MIP-1 alpha (250-09, PeproTech), and 100 mg/mouse anti-IL-10 neutralizing antibody (AB-417-NA, R&D Systems) once. n = 12 regular mice per group. (B) Survival rate. CDI reduced survival rate, which was prevented by citrulline treatment. n = 6 regular mice per group. (C, left panel) Images of H&E-stained colonic tissues in infected mice. 200 mm scale bars are shown in the upper left corners. (C, right panel) Histology scores. Citrulline reduced CDI-dependent colonic injury, which was reversed by MIP-1 alpha but not anti-IL-10 neutralizing antibody injection. n = 6 regular mice per group (mean ± SD). One-way ANOVAs were used. (D) Colonic tissues were collected on day 3 post-infection. Total RNA was extracted and processed for real-time RT-PCR, as described in the Materials and Methods section. Colonic Ccl3 and Il10 mRNA expression were determined by real-time RT-PCR. n = 6 regular mice per group (mean ± SD). One-way ANOVAs were used. (E–F) Fecal samples were collected on day 3 post-infection and suspended in ice-cold PBS. After centrifugation at 2000 g for 5 minutes to remove debris, the clear supernatants were used for citrulline assay and C. difficile toxin ELISAs. Fecal citrulline and C. difficile toxin levels. Oral citrulline treatment increased fecal citrulline but not toxin levels in the infected regular mice. n = 6 regular mice per group (mean ± SD). One-way ANOVAs were used. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39923847), licensed under a CC-BY license. Not internally tested by R&D Systems.