Human CCL27/CTACK Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB3761R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), Immunohistochemistry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 124302
Product Specifications
Immunogen
E. coli-derived recombinant human CCL27/CTACK
Phe25-Gly112
Accession # NP_006655
Phe25-Gly112
Accession # NP_006655
Specificity
Detects human CCL27/CTACK in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse CCL27, recombinant human (rh) CCL25, or rhCCL19 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human CCL27/CTACK Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
ELISA Capture (Matched Antibody Pair)
Optimal dilution of this antibody should be experimentally determined.
ELISA Detection (Matched Antibody Pair)
Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: CCL27/CTACK
CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature human CCL27 is an 88 amino acid (aa) protein that shares 57% aa sequence identity with mouse and rat CCL27 (2). It shares 11%‑35% aa sequence identity with other human CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is up‑regulated by inflammatory stimuli and in wounded skin (4‑7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7‑9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10‑12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA+ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13‑15).
Alternate Names
CTACK, ILC
Gene Symbol
CCL27
UniProt
Additional CCL27/CTACK Products
Product Specific Notices for Human CCL27/CTACK Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
Loading...
Loading...
Loading...