Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Human FAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126) for for the indicated times. PVDF membrane was probed with 1 µg/mL of Human Caspase-10 Monoclonal Antibody (Catalog # MAB834), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase-10 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human Caspase-10 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with Human FAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-10

Caspase-10 (Cysteine-aspartic acid protease 10, FLICE2 and Mch4) is a 28-29 kDa member of the peptidase C14A family of enzymes. It is widely expressed, being found in blood and epithelial cells. Caspase-10 appears to be an initiator caspase that acts on Pro-Caspases-3, -4, -6, -7, and -9. Human Pro-Caspase-10a is a 59 kDa, 521 amino acid (aa) protein. It contains two N-terminal death domains. Cleavage generates p43 plus the small mature p12 subunit. Cleavage of p43 generates a prodomain p25 and large mature p17 subunit. P17 and p12 noncovalently associate to form a 29 kDa mature heterodimer. There are at least four isoform variants. There is no apparent mouse Casp-10 gene.

Alternate Names

CASP10, Caspase10, FLICE2, Mch4

Entrez Gene IDs

843 (Human)

Gene Symbol

CASP10

UniProt

AAC50644

Additional Caspase-10 Products

Product Documents for Human Caspase-10 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Caspase-10 Antibody

For research use only

Citations
Reviews

Human Caspase-10 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB834

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Caspase-10 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Caspase-10 Antibody

Detection of Human Caspase‑10 by Western Blot.