Human Carbohydrate Sulfotransferase 1/CHST1 Alexa Fluor™ Plus 555-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5316AFP555
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 1/CHST1
Specificity
Detects human Carbohydrate Sulfotransferase 1/CHST1 in direct ELISAs and Western blots. In Western blots, approximately 50% cross‑reactivity with recombinant mouse (rm) CHST1 is observed and less than 5% cross‑reactivity with recombinant human (rh) CHST2, rhCHST5, and rmCHST7 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Carbohydrate Sulfotransferase 1/CHST1
The CHST family is comprised of 14 enzymes in humans. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST1, also known as keratan sulfate Gal-6 sulfotransferase, transfers sulfate to position 6 of galactose residues on keratan sulfate (3). It also has sulfotransferase activity on sialyl N-acetyllactosamine structures and participates in biosynthesis of selectin ligands that play a central role in lymphocyte homing at sites of inflammation (4). Human CHST1 shares 94% amino acid sequence identity with mouse CHST1.
References
- Hemmerich, S. and S.D. Rosen (2000) Glycobiology 10:849.
- Bowman, K.G. and C.R. Bertozzi (1999) Chem. Biol. 5:447.
- Fukuta, M. et al. (1997) J. Biol. Chem. 272:32321.
- Yamada, T. et al. (2004) Biochem. J. 384:567.
Alternate Names
6-O-sulfotransferase 1, CHST1, GST-1, KS6ST, KSGal6ST, KSST
Gene Symbol
CHST1
UniProt
Additional Carbohydrate Sulfotransferase 1/CHST1 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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