Human Arylsulfatase A/ARSA Alexa Fluor™ Plus 555-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB2485AFP555
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 324802
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Arylsulfatase A/ARSA
Specificity
Detects human Arylsulfatase A/ARSA in direct ELISAs and Western blots. In direct ELISAs and Western blots, 20‑50% cross‑reactivity with recombinant mouse ARSA is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications
Application
Recommended Usage
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Arylsulfatase A/ARSA
As a member of the sulfatase family, ARSA is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet (1). The ARSA deficiency results in metachromatic leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms (2). The deduced amino acid sequence of human ARSA consists of a signal peptide (residues 1-18) and a mature chain (residues 19-507) (3). Human ARSA has a predicted molecular mass of approximately 53 kDa and an apparent molecular mass of approximately 63 kDa in SDS-PAGE. The amino acid sequence of human ARSA shares 92%, 89%, 87%, and 86% identity with porcine, canine, mouse, and rat ARSA.
References
- Lukatela, G. et al. (1998) Biochemistry 37:3654.
- Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
- Stein, C. et al. (1989) J. Biol. Chem. 264:1252.
Alternate Names
ARSA, Cerebroside 3-sulfatase
Gene Symbol
ARSA
UniProt
Additional Arylsulfatase A/ARSA Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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