Human ALK/CD246 Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4210G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ALK/CD246
Val19-Ser1038
Accession # Q9UM73
Val19-Ser1038
Accession # Q9UM73
Specificity
Detects human ALK/CD246 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human ALK/CD246 Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: ALK/CD246
ALK (Anaplastic Lymphoma Kinase; also CD246) is a 200-220 kDa member of the Insulin receptor subfamily, tyrosine kinase family, protein kinase superfamily of proteins. It shows restricted expression, being limited to select sympathetic and sensory neurons, endothelial cells and tumor cells. Although activation of ALK appears to induce cell proliferation, the exact ligand(s) for ALK is unknown. In a temporally-regulated context, midkine has been suggested to bind to ALK, and ionic Zn has been reported to activate ALK cytoplasmically. Mature human ALK is a 1602 amino acid (aa) type I transmembrane glycoprotein (SwissProt #:Q9UM73). It contains a 1020 aa extracellular region (aa 19-1038) that contains one protein-protein MAM domain (aa 264-427), an LDL receptor class A region (aa 437-473), a second MAM domain (aa 480-631) and one utilized phosphorylation site at Ser211. This is accompanied by a long 561 aa cytoplasmic domain that possesses a protein kinase domain (aa 1116-1392) plus eleven utilized Tyr phosphorylation sites. There is one potential splice variant that contains a four aa insert after Glu549. There is also an 80 kDa soluble product that arises from cleavage of the extracellular domain. Although a third 140 kDa product is frequently seen in SDS-Page, its structural basis is unclear. Almost 20 distinct ALK fusion products arise from gene translocations involving multiple intracellular molecules. These seem to involve blocks of aa starting between aa 1050-1060 and continuing to aa 1620. Over aa 19-1038, human ALK shares 88% aa sequence identity with mouse ALK.
Long Name
Anaplastic Lymphoma Kinase
Alternate Names
CD246, Ki-1, Tcrz
Gene Symbol
ALK
UniProt
Additional ALK/CD246 Products
Product Specific Notices for Human ALK/CD246 Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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