Detects human ALK‑7 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant rat ALK7 is observed and no cross‑reactivity with recombinant mouse Activin RIA/ALK2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human ALK-7 Antibody

ALK‑7 in Human Pancreas.

ALK-7 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human ALK-7 Monoclonal Antibody (Catalog # MAB7749) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human ALK-7 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Reviewed Applications

Read 1 review rated 5 using MAB7749 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK-7

Activin receptor-like kinase 7 (ALK-7), also known as Activin R1C (gene name ACVR1C), is a glycosylated 58 kDa type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. It associates with type II receptors to form a signaling complex that responds to the ligands Activin AB, and Activin B, GDF3, and Nodal. ALK-7 plays a role in regulating energy balance by inhibiting insulin secretion and inducing pancreatic beta cell apoptosis. It is expressed in adipose tissue but downregulated in obesity. ALK-7 is also expressed in pituitary gonadotropic cells and in pre-eclamptic placenta. It induces the apoptosis of trophoblasts as well as ovarian granulosa and epithelial cells. Within the extracellular domain, human ALK-7 shares 95% and 91% amino acid (aa) sequence identity with mouse and rat ALK-7, respectively. Alternate splicing of human ALK-7 generates additional isoforms with either a 50 aa N-terminal truncation or with deletions of 79 aa or 157 aa that encompass the transmembrane segment.

Long Name

Activin Receptor-like Kinase 7

Alternate Names

Activin RIC, ACVR1C, ACVRLK7, ALK7

Entrez Gene IDs

130399 (Human); 245921 (Rat)

Gene Symbol

ACVR1C

UniProt

Q8NER5

Additional ALK-7 Products

Product Documents for Human ALK-7 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Human ALK-7 Antibody

For research use only

Citations
Reviews

Human ALK-7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7749

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ALK-7 Antibodies »

Product Specifications

Immunogen

Specificity