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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 603102

Product Specifications

Immunogen

E. coli-derived recombinant human AIF-1/Iba1
Met1-Pro147
Accession # P55008

Specificity

Detects human AIF-1/Iba1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human AIF-1/Iba1 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AIF-1/Iba1

Allograft-inflammatory factor 1 (AIF-1), also known as daintain and Iba1, is a 17 kDa cytoplasmic protein that contains two calcium-binding EF hand motifs (aa 45-80 and aa 81-115). AIF-1 is expressed in activated macrophages and is important for the proinflammatory response to ischemia, tissue injury, and atherosclerosis. It is also expressed in vascular smooth muscle and endothelial cells in these areas where it promotes vascular remodeling and new vessel formation. AIF-1 is upregulated in allografted tissue during transplant rejection. It also promotes the proliferation of breast cancer cells and in vivo tumorigenesis. The 147 amino acid (aa) isoform 3 of human AIF-1 shares 89% aa sequence identity with mouse and rat AIF-1. Isoform 1 of human AIF-1 lacks the N-terminal 54 aa of isoform 3. Isoform 2 lacks
aa 1-54 as well as aa 121-147 but also has a 66 aa insertion at position 65.

Long Name

Allograft Inflammatory Factor 1

Alternate Names

AIF1, IBA1, IRT1

Entrez Gene IDs

199 (Human); 11629 (Mouse); 29427 (Rat)

Gene Symbol

AIF1

UniProt

Additional AIF-1/Iba1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human AIF-1/Iba1 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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