Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human AFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4558) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AFAP at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human AFAP Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AFAP

AFAP (actin filament associated protein; also known as AFAP-110) is an adaptor protein that regulates actin filament integrity. It is both multimodular and multimeric, and is known to simultaneously bind actin and cytosolic kinases. As a homomultimer, AFAP binds and separates actin filaments, preventing actin cross-linking. Upon binding to kinases, AFAP is destabilized, promoting actin polymerization. Human AFAP is 730 amino acids (aa) in length, and contains SH3 and SH2 binding motifs (aa 69‑74 and aa 91‑96), two pleckstrin homology domains (aa 153‑248 and aa 347‑450) and a Leu-zipper/actin binding region (aa 511‑637). Over aa 450‑730, human AFAP is 86% identical to mouse and rat AFAP.

Long Name

Actin Filament-associated Protein, 110 kDa

Alternate Names

AFAP-110, AFAP1

Entrez Gene IDs

60312 (Human); 70292 (Mouse)

Gene Symbol

AFAP1

UniProt

Q8N556

Additional AFAP Products

Product Documents for Human AFAP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human AFAP Antibody

For research use only

Citations
Reviews

Human AFAP Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4558

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all AFAP Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human AFAP Antibody

Detection of Human AFAP by Western Blot.