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Human 5T4 Antibody

Catalog # AF4975 | R&D Systems, Inc. a Bio-Techne Brand
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AF4975
AF4975-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Product Summary for Human 5T4 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human 5T4
Ser31-Ser355
Accession # Q13641

Specificity

Detects human 5T4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse 5T4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human 5T4 Antibody

Detection of Human 5T4 antibody by Western Blot.

Detection of Human 5T4 by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4975) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for 5T4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
5T4 antibody in Human Placenta by Immunohistochemistry (IHC-P).

5T4 in Human Placenta.

5T4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Sheep Anti-Human 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4975) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
5T4 antibody in Human Placenta by Immunohistochemistry (IHC-P).

5T4 in Human Placenta.

5T4 was detected in immersion fixed paraffin-embedded sections of human placenta using Sheep Anti-Human 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4975) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human 5T4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Simple Western

50 µg/mL
Sample: Human placenta

Western Blot

1 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 3.7 using AF4975 in the following applications:

Published Applications

Read 1 publication using AF4975 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5T4

Human 5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium, and embryonic stem cells and impacts cell adhesion and motility. The human 5T4 cDNA encodes a type I transmembrane protein precursor that is 420 amino acids (aa) in length. It contains a 324 aa extracellular region (aa 32-355) that shows one Ser-rich region followed by seven Leu-rich repeats (aa 90-355). Over aa 31-355, human 5T4 shares 81% and 85% aa identity with mouse and canine 5T4, respectively.

Long Name

Oncofetal Antigen/Trophoblast Glycoprotein

Alternate Names

M6P1, TPBG

Entrez Gene IDs

7162 (Human); 21983 (Mouse); 83684 (Rat); 102132149 (Cynomolgus Monkey)

Gene Symbol

TPBG

UniProt

Product Documents for Human 5T4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human 5T4 Antibody

For research use only

Citations for Human 5T4 Antibody (1)

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Customer Reviews for Human 5T4 Antibody (3)

3.7 out of 5
3 customer ratings
5 stars
0%
4 stars
67%
3 stars
33%
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1 star
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  • Human 5T4 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 06/10/2020
    Human 5T4 Antibody AF4975
  • Human 5T4 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human 5T4 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018

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Reconstitution Calculator

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