HPV16 E7 Antibody (3D6F1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51548

Novus Biologicals, part of Bio-Techne
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NBP1-51548
NBP1-51548-0.025ml
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3D6F1

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all HPV16 E7 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of HPV16 E7 expressed in E. Coli.

Reactivity Notes

Human papillomavirus type 16

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HPV16 E7 Antibody (3D6F1)

Western Blot: HPV16 E7 Antibody (3D6F1)BSA Free [NBP1-51548]

Western Blot: HPV16 E7 Antibody (3D6F1)BSA Free [NBP1-51548]

Western Blot: Papillomavirus 16 E7 Antibody (3D6F1) [NBP1-51548] - Western blot analysis using E7 mouse mAb against truncated GST-E7 recombinant protein (1).

Applications for HPV16 E7 Antibody (3D6F1)

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:500-1:2000

Reviewed Applications

Read 1 review rated 4 using NBP1-51548 in the following applications:

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HPV16 E7

The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15kDa), zinc binding phosphoproteins which are localised in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. The next domain, up to amino acid 37, is homologous to the entire region 2 (CR2) of E1A. Genetic studies have established that these domains are required for cell transformation in vitro, suggesting similarities in the mechanism of transformation by these viruses. The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumour suppressor protein pRb. This sequence is also present in SV40 and polyoma large T antigens. The high risk HPV E7 proteins (of, for example, types 16 and 18) have an approximately ten-fold higher affinity for pRb protein than the low risk HPV E7 proteins (of, for example, type 6). Association of the E7 protein with pRb promotes cell proliferation by the same mechanism as the E1A proteins of adenoviruses and SV40 large T antigen. Recent studies have shown that E7 promotes degradation of Rb family proteins rather than simply inhibiting their function by complex formation. The CR2 region also contains the casein kinase II phosphorylation site (residues 31 and 32). Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.

Alternate Names

E7, HPV16 E7, HpV16gp2, Links Transforming protein human papillomavirus 16, Protein E7, Transforming protein, Transforming protein human papillomavirus 16

Additional HPV16 E7 Products

Product Documents for HPV16 E7 Antibody (3D6F1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HPV16 E7 Antibody (3D6F1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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