Key Product Details

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Western Blot

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 412811

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HO-1/HMOX1/HSP32 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HO-1/HMOX1
Met1-Thr261
Accession # P09601

Specificity

Detects endogenous human and mouse HO-1 in Western blots. In Western blots, this antibody does not cross‑react with rhHO-2.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for HO-1/HMOX1/HSP32 Antibody (412811) [mFluor Violet 450 SE]

Application
Recommended Usage

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HO-1/HMOX1/HSP32

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Long Name

Heme Oxygenase 1

Alternate Names

HMOX1, HO1, HSP32

Gene Symbol

HMOX1

Additional HO-1/HMOX1/HSP32 Products

Product Documents for HO-1/HMOX1/HSP32 Antibody (412811) [mFluor Violet 450 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HO-1/HMOX1/HSP32 Antibody (412811) [mFluor Violet 450 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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