HIV-1 Gag p24 Antibody

Detection of HIV-1 Gag p24 by Western Blot.

Western blot shows recombinant HIV Gag p24 (5 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB7360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIV-1 Gag p24 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of HIV-1 Gag p24 by Western Blot

Effect of ZCCHC3 on viral production(A) Effect of ZCCHC3 on HIV-1 viral production. A plasmid encoding HA-tagged ZCCHC3 or empty HA-vector was introduced into Lenti-X 293T cells together with an HIV-1-encoding plasmid. Virions released into the culture medium were quantified by p24 ELISA. The absolute value (top) and the value relative to that without ZCCHC3 (bottom) are shown (n = 3).(B) ZCCHC3 affects the Gag processing. Lenti-X 293T cells were co-transfected with pTF2625 plasmids in the presence or absence of HA-ZCCHC3. Equal volumes of the pelleted virions and cell lysates were analyzed via western blotting using anti-p24 and anti-HA antibodies. The total protein in the cell lysate was also analyzed via CBB staining.(C) Effect of ZCCHC3 on infectivity of ZCCHC3-loaded virions. TZM-bl cells were infected with the same amount (p24-normalized) of HIV-1NL4-3 or lentivirus with or without ZCCHC3 in the virion. Infectivity was analyzed by luciferase assay and is presented relative to that without ZCCHC3 as the mean and standard deviation (n = 5).(D) Effect of ZCCHC3 co-expression on lentivirus production. HEK293T cells were transfected with lentiviral plasmids (pLV-EGFP, psPAX2, and pMD2.G) with or without an HA-ZCCHC3 expression plasmid. Total RNA was purified from lentiviruses harvested from the culture medium and analyzed by RT-qPCR. The mean and standard deviation values are shown (n = 3). In (A), (C), and (D), differences were examined by a two-tailed, unpaired Student’s t test; ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.001, ∗p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S2589004224003286), licensed under a CC-BY license. Not internally tested by R&D Systems.