Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [PerCP]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30091PCP

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, C. elegans, Drosophila, Mammal, Yeast

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Dot Blot

Label

PerCP (Excitation = 490 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5E10-D8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H4 Antibodies »

Product Specifications

Immunogen

Branched peptide found between amino acids 10-100 of Histone H4. [UniProt# P62805]

Reactivity Notes

All mammals including human, mouse and rat, Drosophila, C. elegans, yeast.

Modification

Monomethyl Lys20

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [PerCP]

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Histone H4 [Monomethyl Lys20] (5E10-D8) antibody is useful in Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence and Western blot. In WB a band is seen at ~11 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Histone H4

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Gene Symbol

HIST4H4

Additional Histone H4 Products

Product Documents for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [PerCP]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [PerCP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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