Histone H3 [Dimethyl Lys9] Antibody - BSA Free

Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200]

Western Blot: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.

Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200]

Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - Mouse NIH3T3 cells were stained with the antibody against H3K9me2 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9me2 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody [NBP2-59200] - ChIP assays were performed using human HeLa cells, the antibody against H3K9me2 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for promoter of the inactive HBB gene and the coding region of the inactive MYOD gene, used as positive controls, and for the promoters of the active genes c-fos and GAPDH, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).