Histone H3 [ac Lys9] Antibody - BSA Free

Western Blot: Histone H3 [ac Lys9] Antibody [NBP2-59180]

Western Blot: Histone H3 [ac Lys9] Antibody [NBP2-59180] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K9ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.

Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody [NBP2-59180] - ChIP assays were performed using human HeLa cells, the antibody against H3K9ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers speci c for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that acetylation of K9 at histone H3 is associated with the promoters of active genes.

Immunofluorescence: Histone H3 [ac Lys9] Antibody [NBP2-59180]

Immunofluorescence: Histone H3 [ac Lys9] Antibody [NBP2-59180] - HeLa cells were stained with the antibody against H3K9ac and with DAPI. Cells were mixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immuno uorescently labelled with the H3K9ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.