HIST1H3A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16838
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding di-methyl Lys4 of human HIST1H3A. The exact sequence is proprietary.
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit HIST1H3A Antibody (NBP2-16838) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for HIST1H3A Antibody
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16838]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16838-img0002.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16838]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16838]
Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16838] - Immunohistochemical analysis of paraffin-embedded Lung AdCA, using antibody at 1:250 dilution.
Chromatin Immunoprecipitation (ChIP): HIST1H3A Antibody [NBP2-16838] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-HIST1H3A antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16838]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16838-img0003.jpg "Immunoprecipitation: HIST1H3A Antibody [NBP2-16838]")
Immunoprecipitation: HIST1H3A Antibody [NBP2-16838]
Immunoprecipitation: HIST1H3A Antibody [NBP2-16838] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Histone H3 (di-methyl Lys4) protein by 2 ug of Histone H3 (di-methyl Lys4) antibody 15% SDS-PAGE The immunoprecipitated Histone H3 (di-methyl Lys4) protein was detected by Histone H3 (di-methyl Lys4) antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.
Applications for HIST1H3A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
10 - 1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:500-1:1000
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HIST1H3A
Alternate Names
H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Gene Symbol
H3C1
Additional HIST1H3A Products
Product Specific Notices for HIST1H3A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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