HIPK2 Antibody (3Z5P6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16825
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3Z5P6 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HIPK2 (Q9H2X6). STSVTGQVLGGPHNLMRRSTVSLLDTYQKCGLKRKSEEIENTSSVQIIEEHPPMIQNNASGATVATATTSTATSKNSGSNSEGDYQLVQHEVLCSMTNTYE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit HIPK2 Antibody (3Z5P6) (NBP3-16825) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for HIPK2 Antibody (3Z5P6)
![Western Blot: HIPK2 Antibody (3Z5P6) [NBP3-16825]](https://resources.bio-techne.com/images/products/HIPK2-Antibody-3Z5P6-Western-Blot-NBP3-16825-img0002.jpg "Western Blot: HIPK2 Antibody (3Z5P6) [NBP3-16825]")
Western Blot: HIPK2 Antibody (3Z5P6) [NBP3-16825]
Western Blot: HIPK2 Antibody (3Z5P6) [NBP3-16825] - Western blot analysis of extracts of various cell lines, using HIPK2 Rabbit mAb (NBP3-16825) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunocytochemistry/ Immunofluorescence: HIPK2 Antibody (3Z5P6) [NBP3-16825]](https://resources.bio-techne.com/images/products/HIPK2-Antibody-3Z5P6-Immunocytochemistry-Immunofluorescence-NBP3-16825-img0001.jpg "Immunocytochemistry/ Immunofluorescence: HIPK2 Antibody (3Z5P6) [NBP3-16825]")
Immunocytochemistry/ Immunofluorescence: HIPK2 Antibody (3Z5P6) [NBP3-16825]
Immunocytochemistry/Immunofluorescence: HIPK2 Antibody (3Z5P6) [NBP3-16825] - Immunofluorescence analysis of U-2 OS cells using HIPK2 Rabbit mAb (NBP3-16825) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: HIPK2 Antibody (3Z5P6) [NBP3-16825] -
Immunocytochemistry/ Immunofluorescence: HIPK2 Antibody (3Z5P6) [NBP3-16825] - Confocal imaging of HeLa cells using HIPK2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.Applications for HIPK2 Antibody (3Z5P6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HIPK2
Long Name
Homeodomain Interacting Protein Kinase 2
Alternate Names
PRO0593, Stank
Gene Symbol
HIPK2
Additional HIPK2 Products
Product Documents for HIPK2 Antibody (3Z5P6)
Product Specific Notices for HIPK2 Antibody (3Z5P6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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