HGFR/c-MET Antibody (1G7NB) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44309
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (91%), Canine (92%), Equine (91%), Feline (92%), Mouse (90%), Porcine (92%), Primate (100%), Rabbit (90%), Rat (90%), Sheep (91%). Backed by our 100% Guarantee.
Applications
Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1G7NB
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant human c-Met protein (between amino acids 170-400) [UniProt P08581].
Localization
Transmembrane Protein with Extracellular, Helical and Cytoplasmic Domains
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for HGFR/c-MET Antibody (1G7NB) - Azide and BSA Free
![Immunocytochemistry/ Immunofluorescence: HGFR/c-MET Antibody (1G7NB) - Azide and BSA Free [NBP2-44309]](https://resources.bio-techne.com/images/products/HGF-R-c-MET-Antibody-1G7NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-44309-img0003.jpg "Immunocytochemistry/ Immunofluorescence: HGFR/c-MET Antibody (1G7NB) - Azide and BSA Free [NBP2-44309]")
Immunocytochemistry/ Immunofluorescence: HGFR/c-MET Antibody (1G7NB) - Azide and BSA Free [NBP2-44309]
Immunocytochemistry/Immunofluorescence: HGF R/c-MET Antibody (1G7NB) - Azide Free [NBP2-44309] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with c-MET (1G7NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (1G7NB) - Azide and BSA Free [NBP2-44309]](https://resources.bio-techne.com/images/products/HGF-R-c-MET-Antibody-1G7NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-44309-img0004.jpg "Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (1G7NB) - Azide and BSA Free [NBP2-44309]")
Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (1G7NB) - Azide and BSA Free [NBP2-44309]
Immunohistochemistry-Paraffin: HGF R/c-MET Antibody (1G7NB) - Azide Free [NBP2-44309] - IHC analysis of HGF R/c-Met (1G7NB) in human liver cancer.
Flow (Intracellular): HGF R/c-MET Antibody (1G7NB) [Azide Free] [NBP2-44309] - c-MET antibody was tested in HeLa cells (1 x 10^6 cells/ml). After fixation and permeabilization, cells were stained using the anti-c-MET antibody (clone 1G7NB) at a 1:1000 dilution. Signal was detected using a Goat anti-Mouse Dylight 488 secondary (blue peak). Shown with secondary control (orange peak). Data was acquired on BD FACSCalibur.
Applications for HGFR/c-MET Antibody (1G7NB) - Azide and BSA Free
Application
Recommended Usage
Flow (Intracellular)
1:1000
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
5-10 ug/ml
Immunohistochemistry-Paraffin
5-10 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HGFR/c-MET
Long Name
Hepatocyte Growth Factor Receptor
Alternate Names
c-MET, cMET, HGF R, MET
Gene Symbol
MET
Additional HGFR/c-MET Products
Product Documents for HGFR/c-MET Antibody (1G7NB) - Azide and BSA Free
Product Specific Notices for HGFR/c-MET Antibody (1G7NB) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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