Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

mFluor Violet 610 SE (Excitation = 421 nm, Emission = 613 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 927312

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Hexokinase 1/2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Hexokinase 2.
Phe11-Arg917
Accession # P52789

Specificity

Detects human Hexokinase 2 in direct ELISAs. Detects human Hexokinase 1 and human Hexokinase 2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Hexokinase 1/2 Antibody (927312) [mFluor Violet 610 SE]

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Hexokinase 1/2

An alteration in the permeability of the mitochondrial membrane is a hallmark of the intrinsic pathway of caspase activation. This change in permeability leads to the cytosolic release of multiple proteins, including cytochrome c, Smac/Diablo, HTRA2/Omi, Apoptosis Inducing Factor (AIF), and Endonuclease G from the intermembrane space of the mitochondria. The release of these proteins, which are normally sequestered, has severe consequences for the cell. For example, cytoplasmic cytochrome c and dATP initiate the assembly of the apoptosome with APAF-1 and pro-caspase-9. The assembly of this complex promotes the activation of caspase-9 which subsequently leads to the activation of caspase-3, -6, and -7 to induce apoptosis. In addition, Smac/Diablo and HTRA2/Omi interact with the IAP proteins disrupting their abilities to inhibit caspase activity, while AIF and Endonuclease G translocate to the nucleus upon mitochondrial release and promote chromatin condensation and DNA fragmentation.

Additional Hexokinase 1/2 Products

Product Documents for Hexokinase 1/2 Antibody (927312) [mFluor Violet 610 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Hexokinase 1/2 Antibody (927312) [mFluor Violet 610 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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