Key Product Details

Species Reactivity

HCoV-HKU1

Applications

Immunocytochemistry

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 1045307

View all Spike RBD Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell HEK293-derived HCoV-HKU1 Spike RBD
Thr310-Tyr624
Accession # Q5MQD0.1

Specificity

Detects Human Coronavirus HCoV-HKU1 Spike RBD and 229E in direct ELISAs. No staining of NL63 HKu1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Spike RBD

HCoV-HKU1 was identified in Hong Kong in 2005 as a new human coronavirus (1). Coronaviruses are a family of viruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein(N). There are two well-known human coronavirus families that infect humans: Alpha coronaviruses which includes HCoV-229E and HCoV-NL63; beta coronaviruses that includes HCov-OC43, Severe Acute Respiratory Syndrome (SARS-CoV), Middle East Respirator Syndrome (MERS-CoV), and global pandemic Covid-19 (SARS-CoV2) (2). The HCoV-HKU1 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. As with most coronaviruses, proteolytic cleavage of the S protein generates two distinct peptides, S1 and S2 subunits. The S1 subunit is focused on attachment of the protein to the host receptor, while the S2 subunit is involved with cell fusion. The receptor binding domain (RBD) of HCoV-HKU1 is located at C-terminal region of S1 subunit, similar to SARS-COV, MERS-COV and SARS-COV2, but the RBD regions do not share significant amino acid sequence identity (3). HCoV-HKU1 has been demonstrated to bind specifically to 9-O-acetylated sialic acids (9-O-Ac-Sias) attached as terminal residues to glycan chains on glycoproteins and lipids, but additional receptors remain unknown (4). HCoV-HKU1, along with HCov-OC43, differ from other cornonaviruses in that their virions possess two types of surface projections, both involved in attachment: large "spikes" that are comprised of homotrimers of the S protein, and unique, smaller protrusions, comprised of the homodimeric hemagglutinin-esterase (HE) (5).

Long Name

Spike Receptor Binding Domain

Entrez Gene IDs

3200426 (HCoV-HKU1); 14254594 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

Q5MQD0.1

Additional Spike RBD Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.