GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25620
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation, Independent Antibodies, Tagged Protein Expression
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1864 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human GPRC5A/RAI3.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free
Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] -
Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] -
Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. RAI3 stained by RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] -
Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications for GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GPRC5A
GPRC5A is a member of the G protein-coupled receptor family, characterized by a signature 7- transmembrane domain motif. GPRC5A is a lung-specific tumor suppressor and may play a role in the interaction between retinoid acid and G protein signaling pathways. GPRC5A is also involved in embryonic development and epithelial cell differentiation.
Long Name
G Protein-coupled Receptor, Family C, Group 5, Member A
Alternate Names
RAI3, RAIG1
Gene Symbol
GPRC5A
Additional GPRC5A Products
Product Documents for GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free
Product Specific Notices for GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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