GPR3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00196
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-LQRHLLPASH corresponding to internal region according to NP_005272.1.
Specificity
This antibody is not expected to cross-react with GPR6 and GPR12.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Novus Biologicals Goat GPR3 Antibody (NBP1-00196) is a polyclonal antibody validated for use in WB and ELISA. Anti-GPR3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for GPR3 Antibody
![Western Blot: GPR3 Antibody [NBP1-00196]](https://resources.bio-techne.com/images/products/GPR3-Antibody-Western-Blot-NBP1-00196-img0002.jpg "Western Blot: GPR3 Antibody [NBP1-00196]")
Western Blot: GPR3 Antibody [NBP1-00196]
Western Blot: GPR3 Antibody [NBP1-00196] - (0.3ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for GPR3 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
0.3 - 1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in human brain (hippocampus and frontal cortex) lysates (calculated MW of 35.0 kDa band according to NP_005272.1).
An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GPR3
GPR3, previously called ACCA (adenylate cyclase constitutive activator), is 7-transmembrane receptor that has been shown to promote cAMP accumulation. In oocytes, cAMP generated by GPR3 regulates maintenance of meiotic arrest. GPR3 is also expressed in the brain, where it has been implicated in cAMP-dependent emotional responses and modulation of amyloid-beta peptide generation.
Long Name
G Protein-coupled Receptor 3
Alternate Names
ACCA, G protein-coupled receptor 3
Gene Symbol
GPR3
UniProt
Additional GPR3 Products
Product Documents for GPR3 Antibody
Product Specific Notices for GPR3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...