GPR114 Antibody (HL3850)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48850
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL3850
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide encompassing a sequence within the Extracellular domain of human GPR114. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit GPR114 Antibody (HL3850) (NBP3-48850) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for GPR114 Antibody (HL3850)
Immunocytochemistry/ Immunofluorescence: GPR114 Antibody (HL3850) [NBP3-48850] -
GPR114 antibody [HL3850] detects GPR114 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: GPR114 stained by GPR114 antibody [HL3850] (NBP3-48850) diluted at 1:500.Blue: Fluoroshield with DAPI .
Western Blot: GPR114 Antibody (HL3850) [NBP3-48850] -
Non-transfected (-) and transfected (+) unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR114 antibody [HL3850] (NBP3-48850) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for GPR114 Antibody (HL3850)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GPR114
Long Name
G Protein-coupled Receptor 114
Alternate Names
PGR27
Gene Symbol
ADGRG5
Additional GPR114 Products
Product Documents for GPR114 Antibody (HL3850)
Product Specific Notices for GPR114 Antibody (HL3850)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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