GloLIVE Human TRA-1-60(R) NL493 Live Cell Antibody
R&D Systems, part of Bio-Techne | Catalog # NLLC4770G
50X Concentration, Azide Free
Discontinued Product
NLLC4770G has been discontinued.
View all TRA-1-60(R) products.
Key Product Details
Species
Human
Applications
Immunocytochemistry/ Immunofluorescence
Conjugate
NL493
Product Summary for GloLIVE Human TRA-1-60(R) NL493 Live Cell Antibody
Kit Summary
To detect TRA-1-60(R) expression in live cells by immunocytochemical staining.
Why confirm marker expression in live cells before colony selection?
Maintaining pluripotent human embryonic stem cells and deriving induced pluripotent stem cells require colony selection and cell expansion. Picking colonies that contain pluripotent stem cells is typically achieved by manually analyzing colony morphology. Unfortunately, this critical process is time-consuming and labor-intensive.
In addition, morphology-based colony selection does not consistently guarantee pluripotent starting populations, since cells can fail to be fully reprogrammed or can naturally differentiate within a colony.
To address this need, R&D Systems offers GloLIVE™ antibodies that allow researchers to confirm stem cell marker expression in live cells before colony selection.
Live pluripotent stem cell imaging:
- Promotes the selection of high quality, undifferentiated stem cell colonies to reduce experimental variation.
- Verifies stem cell pluripotency in 30 minutes, ensuring efficient use of time and reagents.
- Allows for the continued culture of pluripotent cells with no adverse effects on cell proliferation or stemness following staining.
Antibody Components
- GloLIVE NL493-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody.
- Supplied as 50X concentration of antibody in 0.5 mL PBS
Azide free and endotoxin tested (= 5 EU/mL), this antibody can be used for 12 tests, if a staining volume of 2 mL is used.
Product Specifications
Specificity
Detects human TRA‑1‑60(R).
Clone
TRA-1-60
Scientific Data Images for GloLIVE Human TRA-1-60(R) NL493 Live Cell Antibody
Detection of TRA-1-60(R) in Live BG01V Human Embryonic Stem Cells.
The stem cell marker TRA-1-60(R) was visualized in live BG01V human embryonic stem cells using a GloLIVE NL493-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (Catalog # NLLC4770G; green). The nuclei were counterstained with Hoechst 33342 (blue). Positive staining for TRA-1-60(R) in human stem cells is an indicator of pluripotency.Formulation, Preparation, and Storage
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: TRA-1-60(R)
TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2‑4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).
References
- Andrews, P. et al. (1984) Hybridoma 3:347.
- Thomson, J. et al. (1998) Science 282:1145.
- Giwercman, A. et al. (1993) Cancer 72:1308.
- Marrink, J. et al. (1991) Int. J. Cancer 49:368.
- Badcock, G. et al. (1999) Cancer Res. 59:4715.
- Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.
Long Name
Tumor-related Antigen-1-60 [R]
Alternate Names
TRA160(R)
Entrez Gene IDs
5420 (Human)
Gene Symbol
PODXL
Additional TRA-1-60(R) Products
Product Documents for GloLIVE Human TRA-1-60(R) NL493 Live Cell Antibody
Product Specific Notices for GloLIVE Human TRA-1-60(R) NL493 Live Cell Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...