G9a/EHMT2 Antibody (23GB825)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51973
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB825
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human G9a/EHMT2 (Uniprot: Q96KQ7)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for G9a/EHMT2 Antibody (23GB825)
Immunocytochemistry/ Immunofluorescence: G9a/EHMT2 Antibody (23GB825)[NBP3-51973] -
G9a/EHMT2 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: EHMT2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um. Permeabilization: Triton.
Immunocytochemistry/ Immunofluorescence: G9a/EHMT2 Antibody (23GB825)[NBP3-51973] -
Immunocytochemical staining of HepG2 cells with G9a/EHMT2 antibody (1:1,000). Nuclei were stained blue with DAPI; G9a/EHMT2 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
Flow Cytometry: G9a/EHMT2 Antibody (23GB825)[NBP3-51973] -
Validation of G9a/EHMT2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with G9a/EHMT2 antibody (1:2,000) and analyzed using CytoFLEX.Applications for G9a/EHMT2 Antibody (23GB825)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: G9a/EHMT2
Long Name
G9a Histone Methyltransferase/Euchromatic Histone-lysine N-methyltransferase 2
Alternate Names
EHMT2
Gene Symbol
EHMT2
Additional G9a/EHMT2 Products
Product Documents for G9a/EHMT2 Antibody (23GB825)
Product Specific Notices for G9a/EHMT2 Antibody (23GB825)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...